A0A2V4VLX2 · A0A2V4VLX2_PAEBA

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site31-35NAD+ (UniProtKB | ChEBI)
Binding site79-80NAD+ (UniProtKB | ChEBI)
Binding site120NAD+ (UniProtKB | ChEBI)
Active site122N6-AMP-lysine intermediate
Binding site143NAD+ (UniProtKB | ChEBI)
Binding site178NAD+ (UniProtKB | ChEBI)
Binding site290NAD+ (UniProtKB | ChEBI)
Binding site314NAD+ (UniProtKB | ChEBI)
Binding site408Zn2+ (UniProtKB | ChEBI)
Binding site411Zn2+ (UniProtKB | ChEBI)
Binding site424Zn2+ (UniProtKB | ChEBI)
Binding site430Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      DFQ00_1033

Organism names

  • Taxonomic identifier
  • Strain
    • CECT 7022
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus

Accessions

  • Primary accession
    A0A2V4VLX2

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain591-670BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    670
  • Mass (Da)
    74,995
  • Last updated
    2018-09-12 v1
  • Checksum
    131BECA6B1D48B8B
MDPMHRMEQLVTELNQHNYQYYTMDQPQISDKEYDLLYDELVTLEQESGMVLPDSPTQRVGGELLKGFTPHRHLSPLWSLDKAQNIEQLRNWNTRVLKLVNDYNSKNPESPLPDPGYVIELKFDGLTLNLTYTNGELVQASTRGNGTVGEGILAQVRTIKSVPLKIPYTGGTIEVQGEGIMNLSVLNQYNETAAEPLKNARNAAAGALRNLNPKTTAERRLNAYFYNIGYSDDIQFATHQEMMDFLRENRFKVNPSITYFHEFDDVMEQLAAIQENRGQLDYLIDGAVIKLTDMRTREVLGYTDKFPRWAVAYKFEAEETTTVLNEVVWNVGRTGKVTPLARVEAVELAGVTVQNCTLNNVGDIERKNLKFALGSRVFIRRSNDVIPEILGKVTEENDGEEIIYPENCPACGFPLEQRGAHLFCNNKLACKPQTVARISHFASRDAMDIETFSEKTAIQLYDELNVREPADLYTLQFDDLVKLERFGEKKANNLLAALEQSKDRDLASFLYSLGIPNTGKSTTRMLAEHYRDLHAIMNATVEELIELPDVGGIVAESIVNFFADPFTQAAIEKMLNLGVKAQAPEAPAVVAEDSFFSGKTVVLTGTLHQLTREEATQRLEALGAKVTGSVSKKTDLVIAGEKAGSKLTKARDLGIPTIEDEDELVRLLNS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QJSW01000003
EMBL· GenBank· DDBJ
PYE50586.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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