A0A2T5M393 · A0A2T5M393_9EURO
- Proteinalpha-1,2-Mannosidase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids492 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activity
- 3 H2O + N4-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 beta-D-mannose + N4-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)
- 4 H2O + N4-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 beta-D-mannose + N4-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)
Cofactor
Protein has several cofactor binding sites:
Pathway
Protein modification; protein glycosylation.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasmic vesicle lumen | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | mannosyl-oligosaccharide 1,2-alpha-mannosidase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | ERAD pathway | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namealpha-1,2-Mannosidase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionA0A2T5M393
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MRLHLLALPATGLALVGSPSGSQA | ||||||
Chain | PRO_5015632310 | 25-492 | alpha-1,2-Mannosidase | |||
Sequence: DTVKEAFSHAWDGYMKYAFPHDELLPLSNGYGDSRNHWGASAVDALSTATIMGNATIVTQILDHIATIDYSQTASTVSLFETTIRYLAGMLSGYDLLKGPAAGLVDDNKVDVLLEQSKNLADVLKFAFDTPSGVPYNNINITSGGNDGSPTNGLAVTGTLVLEWTRLSDLTGDPEYARLSQRAESYLLHPHPASSEPFPGLVGSSISIETGNFTDGSVSWNGGDDSFYEYLIKMYVYDPKRFGVYKDRWVAAAESSMKYLASHPATRADLTFLATYNNGEYGLRSQHLACFDGGNFILGGSVLGRDDLLAFGVELVESCHETYNATVTGIGPEAFSWDSGAVPRAQAAFFDTAGFWIENGAYILRPEVIESFYYAWRITGQEEYREWIWNAFGAINKYCRTPSGFAGLTDVNAANGGSRDNNQESFLFAEVMKYSYLAFAPDDEWQVQRGGGNTFVFNTEAHPFRIST | ||||||
Disulfide bond | 314↔343 | |||||
Sequence: CFDGGNFILGGSVLGRDDLLAFGVELVESC |
Keywords
- PTM
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length492
- Mass (Da)53,672
- Last updated2018-07-18 v1
- Checksum15C75B519280A87E