A0A1Y1JG74 · A0A1Y1JG74_PLAGO
- ProteintRNA (guanine(37)-N1)-methyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids748 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalytic activity
- guanosine37 in tRNA + S-adenosyl-L-methionine = N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteine + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 416 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 454-455 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DI | ||||||
Binding site | 481-482 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Binding site | 650 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Cellular Component | nucleus | |
Molecular Function | tRNA (guanine(37)-N1)-methyltransferase activity | |
Biological Process | tRNA N1-guanine methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(37)-N1)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Plasmodium)
Accessions
- Primary accessionA0A1Y1JG74
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly in the mitochondria and in the nucleus.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-20 | |||||
Sequence: MFIRILYFLWILQHFAYVAS | ||||||
Chain | PRO_5012620951 | 21-748 | tRNA (guanine37-N1)-methyltransferase | |||
Sequence: LRKKGNFLHAFVKMNNPVNIKNLRDVKEKVKYEKKTHCLVLKKSKTNTILKNKYTKCWLMNVFKFPSVLKFVEYQKALIDSGVYNKELMSLIHTYLEKLEVGTRSMKNHASGLTHDQTIDMVKCETADVLKCETDDMVKYATIGQIGDHATDAVINAAGIDEGELRLIPLNEHFNKTLHELMEREGKGIMNGIDISPDEGEFQSDEEKKESASLRRERKYWNLINNEENNIKKEKYQMLKDVNKWPEDSILNDSSNRMKYLTELFYLIKKEDIKLSVIRLEFGYDNMNTSEILKKIFPSGNEIIHKYEMIGHIAHLNFCEKFEDYKKVIAEIILDKNKSIKTVINKKEMLNNVHRTFNMELLAGEKNYLTMLKENDIKIKLNYELIYWNSKLKKERDRIYDLVKKNSIIIDMFGGVGIFSLHLSKKNCLCFSNDINEHAYNYMNINVKLNKRKNILTYNLDATAFIDMLILLNLFSNDASTLTIHLNEQNIKNISLDFINSVHEQTNDNIKKKKSATTGTSNKTPDLVSEKKDAYHDVLDKKIKITSGDTNKFADEQDNNRYICHKDGLEPIHDVQKISQNSLSPQEERASCQLFIKDDAEKAINGELKNDLHKVEINLKHYGDIHVLMNLPQTAFKFLDVFRKIKNIYSVHKTDTQDKYVKDKMRNVFIHCYYFSKPEFFYEDAERNIRLQLKGIPREMKITEIRKVSPSKLMYVVEFNLKDIVGTE |
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 327-743 | SAM-dependent methyltransferase TRM5/TYW2-type | ||||
Sequence: YEMIGHIAHLNFCEKFEDYKKVIAEIILDKNKSIKTVINKKEMLNNVHRTFNMELLAGEKNYLTMLKENDIKIKLNYELIYWNSKLKKERDRIYDLVKKNSIIIDMFGGVGIFSLHLSKKNCLCFSNDINEHAYNYMNINVKLNKRKNILTYNLDATAFIDMLILLNLFSNDASTLTIHLNEQNIKNISLDFINSVHEQTNDNIKKKKSATTGTSNKTPDLVSEKKDAYHDVLDKKIKITSGDTNKFADEQDNNRYICHKDGLEPIHDVQKISQNSLSPQEERASCQLFIKDDAEKAINGELKNDLHKVEINLKHYGDIHVLMNLPQTAFKFLDVFRKIKNIYSVHKTDTQDKYVKDKMRNVFIHCYYFSKPEFFYEDAERNIRLQLKGIPREMKITEIRKVSPSKLMYVVEFNLKD |
Sequence similarities
Belongs to the TRM5 / TYW2 family.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length748
- Mass (Da)87,552
- Last updated2017-08-30 v1
- Checksum7793B9EADA689BE0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BDQF01000008 EMBL· GenBank· DDBJ | GAW80207.1 EMBL· GenBank· DDBJ | Genomic DNA |