A0A168QEH2 · A0A168QEH2_MUCCL
- ProteinNADPH-dependent diflavin oxidoreductase 1
- GeneTAH18
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids580 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.
Catalytic activity
- NADPH + 2 oxidized [2Fe-2S]-[protein] = H+ + NADP+ + 2 reduced [2Fe-2S]-[protein]
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 58-61 | FMN (UniProtKB | ChEBI) | ||||
Sequence: STTG | ||||||
Binding site | 96-105 | FMN (UniProtKB | ChEBI) | ||||
Sequence: LGDSSYRKFN | ||||||
Binding site | 131 | FMN (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 336 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 366-369 | FAD (UniProtKB | ChEBI) | ||||
Sequence: RSFS | ||||||
Binding site | 442 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 498-499 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: SR | ||||||
Binding site | 504-508 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: KVYVQ | ||||||
Binding site | 579 | FAD (UniProtKB | ChEBI) | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Biological Process | iron-sulfur cluster assembly |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent diflavin oxidoreductase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Fungi incertae sedis > Mucoromycota > Mucoromycotina > Mucoromycetes > Mucorales > Mucorineae > Mucoraceae > Mucor
Accessions
- Primary accessionA0A168QEH2
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Relocalizes to mitochondria after H2O2 exposure.
Keywords
- Cellular component
Interaction
Subunit
Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-149 | Flavodoxin-like | ||||
Sequence: LLILFGSETGCAQDVAENLARQARRRHFKTRVVAMDDYDKSQLVEEKLVFFVCSTTGQGIEPANMKKFWKFLLRKNLPNDILSDLDCAVLGLGDSSYRKFNYPSKKLYKRLLQLGANMIVDRGDCDDQHYLGLDGAFVPWAKNLW | ||||||
Domain | 190-428 | FAD-binding FR-type | ||||
Sequence: AEEFNLVVRKNERITAADHFQDVRHIELACTDQDFKYEPGDIAVITPQNLVQDVDLFFEQLGWTEHADKLIRFTPFDEFHTLPAHWPSVMTFRDLFVYFLDVFGVPRRSFFEMLAYFTTDENHTERLREFASPQGQEDMWAYCARPRRHIGEVLFDFKPFDIPFDYMLDLFPQLQPRSFSIASSLNVHPKSMELCVAVVKYKTKMRKIRRGVFTKWMSTWEEGDVIPRVRIAKGTMTLP |
Sequence similarities
Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length580
- Mass (Da)67,097
- Last updated2016-07-06 v1
- Checksum85AABE53748CC8B9
Keywords
- Technical term