A0A0H3MCT1 · A0A0H3MCT1_ECO7I
- ProteinD-alanine--D-alanine ligase
- GeneddlB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids306 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Cell wall formation.
Catalytic activity
- 2 D-alanine + ATP = D-alanyl-D-alanine + ADP + phosphate + H+
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 15 | |||||
Sequence: E | ||||||
Active site | 150 | |||||
Sequence: S | ||||||
Binding site | 257 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 270 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 270 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 272 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 281 | |||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | D-alanine-D-alanine ligase activity | |
Molecular Function | metal ion binding | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-alanine--D-alanine ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A0H3MCT1
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 101-303 | ATP-grasp | ||||
Sequence: KLLWQGAGLPVAPWVTLTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAENALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLSDETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVRILE |
Sequence similarities
Belongs to the D-alanine--D-alanine ligase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length306
- Mass (Da)32,870
- Last updated2015-09-16 v1
- ChecksumFEB50CAE53BC3D14
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU928164 EMBL· GenBank· DDBJ | CAR16236.1 EMBL· GenBank· DDBJ | Genomic DNA |