A0A088RI92 · A0A088RI92_9TRYP
- ProteintRNA 4-demethylwyosine synthase (AdoMet-dependent)
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids845 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- N1-methylguanosine37 in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine37 in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O
RHEA-COMP:10165 CHEBI:73542 Position: 37+ CHEBI:15361 + CHEBI:59789 = RHEA-COMP:10164 CHEBI:64315 Position: 37+ CHEBI:17319 + CHEBI:57844 + CHEBI:16526 + CHEBI:15377
Cofactor
Pathway
tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | FMN binding | |
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | wybutosine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA 4-demethylwyosine synthase (AdoMet-dependent)
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Leishmaniinae > Leishmania > Leishmania guyanensis species complex
Accessions
- Primary accessionA0A088RI92
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-29 | |||||
Sequence: MMIAFLFQLLVSVGLPLVALLLYNHLADS | ||||||
Chain | PRO_5001838969 | 30-845 | tRNA 4-demethylwyosine synthase (AdoMet-dependent) | |||
Sequence: IHARGSVNVDDVNAFSQRVRAELEQLNVPPALPEAAPGQAAPPPAPMRSSPAVTAPPPPEAAEILIAYATQSQNSLALAHKLFSLITAQLHTAPPSTTDDAVCPLVRVMEMREEEDSSASVAAASAAGGRVSRTNVCRVDTLLEQSQYALIIFIASTYTNGVAPPRSQAFEALLKDSFEDHRIPRNTLARKRFAVFGLGDIAYGEERFNAFAKNLHEWCRGLGAPPFVVPPVYTTEAKTQSLFRIFSTALLKWVSRATFHRDGTVTVKKKSEMGTTGPAAASATGMLSHKAGGITAAPAGETCGSEGASCACQQSTQQQQQQRQPCGNSTGPDGDDNCGCCKTDSIANCVAASKRDDDDEADDDDEEEEVNGENDGMDDVEDLVWDGTEDTDFDINKDPSELPELLYPKLRQNLEKQGYRLVGTHSGVKLCRWTKSMLRGRGGCYKHTFYNINSSQCMEMTPSLACANKCVFCWRHHTNPVSRHFRWKQDPPELLIAQGMAGHYQMIKQMRGVPGVTPERLATAMQIRHCALSLVGEPIMYPQINGFCELLHQHHISSFMVTNAQFPEQLRDLKPVVQLYLSIDAPTPEELKRIDRPLFEDYWERCLSCVKELAKKQQRTVFRLTLVNQYNTENVKAYADLVEMGQPDFIEVKGVTYCGTSTSSTLTMKDNVPRHDEVISFCKALCTEMASRHPHYSTLQMNTEEKGGDDGDDDEVIVIPAEERNRPYHVACEHEHSCCVLIALDKFFFEGHWHTWIDYERFYHLVESGRTDFTSLDYAAVTPAWATYNSKEKGFDPEQTRVVRKNARPTVVTASC |
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 56-85 | Disordered | ||||
Sequence: NVPPALPEAAPGQAAPPPAPMRSSPAVTAP | ||||||
Compositional bias | 64-85 | Pro residues | ||||
Sequence: AAPGQAAPPPAPMRSSPAVTAP | ||||||
Domain | 93-287 | Flavodoxin-like | ||||
Sequence: ILIAYATQSQNSLALAHKLFSLITAQLHTAPPSTTDDAVCPLVRVMEMREEEDSSASVAAASAAGGRVSRTNVCRVDTLLEQSQYALIIFIASTYTNGVAPPRSQAFEALLKDSFEDHRIPRNTLARKRFAVFGLGDIAYGEERFNAFAKNLHEWCRGLGAPPFVVPPVYTTEAKTQSLFRIFSTALLKWVSRAT | ||||||
Region | 382-410 | Disordered | ||||
Sequence: SKRDDDDEADDDDEEEEVNGENDGMDDVE | ||||||
Compositional bias | 386-410 | Acidic residues | ||||
Sequence: DDDEADDDDEEEEVNGENDGMDDVE | ||||||
Domain | 479-723 | Radical SAM core | ||||
Sequence: YNINSSQCMEMTPSLACANKCVFCWRHHTNPVSRHFRWKQDPPELLIAQGMAGHYQMIKQMRGVPGVTPERLATAMQIRHCALSLVGEPIMYPQINGFCELLHQHHISSFMVTNAQFPEQLRDLKPVVQLYLSIDAPTPEELKRIDRPLFEDYWERCLSCVKELAKKQQRTVFRLTLVNQYNTENVKAYADLVEMGQPDFIEVKGVTYCGTSTSSTLTMKDNVPRHDEVISFCKALCTEMASRHP |
Sequence similarities
Belongs to the TYW1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length845
- Mass (Da)93,761
- Last updated2014-11-26 v1
- Checksum742926487FCE4D06
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 64-85 | Pro residues | ||||
Sequence: AAPGQAAPPPAPMRSSPAVTAP | ||||||
Compositional bias | 386-410 | Acidic residues | ||||
Sequence: DDDEADDDDEEEEVNGENDGMDDVE |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP009374 EMBL· GenBank· DDBJ | AIN95515.1 EMBL· GenBank· DDBJ | Genomic DNA |