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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)A0A352VXM4[477 - 477]
Description
Feature keySite
Feature identifier
        10         20         30         40         50
MKPGVILVAG TGSGVGKTSI AVGLARALAD RSFTVQPFKT GPDFLDPVWL
60 70 80 90 100
TRAARRSCVS LDGWMCGKSY VCSLAETLSR DADIILVEGV MGMYDGADPG
110 120 130 140 150
NLAGSSAEIA VWLDASVLLV INVHGSVRST AAIARGFADF EPAPGVAMVI
160 170 180 190 200
ANQAGSSSHA DMLATAFAAP GCPPLAGVIP RGALSSLSSR HLGLAAPDHD
210 220 230 240 250
DRGASAAEET IGALASAVEK HIQIDLILSR INIKQDKAVK KINIPPSPSR
260 270 280 290 300
YYSYALGSAP SLLYAPYDKL FLKKPDHNVK IGIALDEAFS FYYPDNLEAI
310 320 330 340 350
KEAGAELVPF SPLHDNCLPE NLDGIYLGGG YPELHAEKLS ENTGIKNAIT
360 370 380 390 400
AFAGTGHTVY AECGGIMYLS RGITTVSGNF FLMCGILPFA VRMSSSLRRL
410 420 430 440 450
GYVSIKTEAD CIWGKAGTEI RGHEFHYSEI DRQNGQPDFT DCSRQGWTRI
460 470 480 490 500
YSAEHTRTGR REPEGFYNGC VLASYVHLHF ASQPACARSF VEFCARKKSL
510 520 530 540 550
KIPPVSSSEN IITLRSGFTT GSAAAAAALA AAVLIAGRKA LNRAEIIIPG
560 570 580 590 600
KSENTGESGI LAIPVAESNL IRPGAAASAV VIKDAGDDPD ITDRVHIRVL
610 620 630 640 650
VEHTEEPGRN ILFQAGEGVG IVTKPGLQIP PGEPAINPVP RAMITDALRS
660 670 680 690 700
VLPEGSWRIT VSAAGGENLA LKTFNPRLGI TGGISILGTT GIVRPFSHSA
710 720 730 740 750
LKSALRCAFN VALASGADPL VLVPGNIGRR AFSAAYPEFA DDHIIDVSNE
760 770 780 790 800
WGTVLEWIHE AKTIKTIAVC GHPGKLTKLL GDSWDTHSSR SPSALPLIFS
810 820 830 840 850
LAREAGINYS FDNITTAEGF FTDIKSDERE KIANLLAQRI CDKIKIRCFC
860 870 880 890 900
ADAAVLLCDM NGKIIGSTGS DIFKRKYSTT AEVSAGGRVS HIKDETRKIV
910 920 930 940 950
IAGCGPGSHN HLTAEVIEAV KNADVLIGTD RVLELFSESK ARKITCNGSI
960 970 980 990 1000
NAVCDIITRE DAHRVCVLVT GDPGFYSLAS LVVRRFGARQ CIILPGITSV
1010 1020 1030 1040 1050
QAAAARLGIS WAHALLLSAH TKIPQDTEIF VNDRELPETL IILGGCKDSP
1060 1070 1080 1090 1100
EWITRLGQRL GSKYRGWLCE NICLPDERIT AFPGVPQSGS VWLVIFSRCA
1110 1120
YNQDVEMSPD TKSSQKESXQ AMKQMG
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