W5KLY9 · W5KLY9_ASTMX
- ProteinHedgehog protein
- GeneSHH
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids419 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.
Protein hedgehog N-product
The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development.
Protein hedgehog
The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product.
Catalytic activity
- glycyl-L-cysteinyl-[protein] + cholesterol + H+ = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein]This reaction proceeds in the forward direction.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 90 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 91 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 91 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 96 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 126 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 127 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 127 | Ca2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 130 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 132 | Ca2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 141 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 148 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 183 | Zn2+ (UniProtKB | ChEBI) | |||
Site | 198-199 | Cleavage; by autolysis | |||
Site | 244 | Involved in cholesterol transfer | |||
Site | 268 | Involved in auto-cleavage | |||
Site | 271 | Essential for auto-cleavage | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | extracellular space | |
Cellular Component | Golgi membrane | |
Cellular Component | plasma membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | patched binding | |
Molecular Function | peptidase activity | |
Molecular Function | transferase activity | |
Biological Process | animal organ development | |
Biological Process | cell development | |
Biological Process | cell fate specification | |
Biological Process | cell-cell signaling | |
Biological Process | central nervous system development | |
Biological Process | intein-mediated protein splicing | |
Biological Process | neuron differentiation | |
Biological Process | pattern specification process | |
Biological Process | protein autoprocessing | |
Biological Process | regulation of cell population proliferation | |
Biological Process | regulation of developmental process | |
Biological Process | regulation of gene expression | |
Biological Process | smoothened signaling pathway | |
Biological Process | tissue development | |
Biological Process | tube development |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameHedgehog protein
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Characiformes > Characoidei > Characidae > Astyanax
Accessions
- Primary accessionW5KLY9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-24 | ||||
Chain | PRO_5017223563 | 25-419 | Hedgehog protein | ||
Keywords
- PTM
Expression
Gene expression databases
Interaction
Subunit
Multimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 197-307 | Hint | |||
Domain | 315-361 | Hint | |||
Sequence similarities
Belongs to the hedgehog family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length419
- Mass (Da)46,375
- Last updated2018-12-05 v2
- MD5 Checksum0DA90D8F554D1EEBEE48EC82723A04FF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A3B1J4Z1 | A0A3B1J4Z1_ASTMX | SHH | 443 |
Keywords
- Technical term