U3FIG2 · U3FIG2_CALJA

Function

function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.

Catalytic activity

  • 1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1,2-didecanoyl-sn-glycerol + ATP = 1,2-didecanoyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1,2-dihexadecanoyl-sn-glycerol + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-O-alkyl-2-acyl-sn-glycerol + ATP = 1-O-alkyl-2-acyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-O-hexadecyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-O-hexadecyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-O-hexadecyl-2-acetyl-sn-glycerol + ATP = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-O-hexadecyl-sn-glycerol + ATP = 1-O-hexadecyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • 2-(9Z-octadecenoyl)-glycerol + ATP = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
  • a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
    EC:2.7.1.107 (UniProtKB | ENZYME | Rhea)
  • a 1-O-alkyl-sn-glycerol + ATP = a 1-O-alkyl-sn-glycero-3-phosphate + ADP + H+
    This reaction proceeds in the forward direction.
    EC:2.7.1.93 (UniProtKB | ENZYME | Rhea)

Pathway

Lipid metabolism; glycerolipid metabolism.

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular FunctionATP-dependent diacylglycerol kinase activity
Molecular Functioncalcium ion binding
Biological Processglycerolipid metabolic process
Biological Processphospholipase C-activating G protein-coupled receptor signaling pathway
Biological Processplatelet activation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Diacylglycerol kinase
  • EC number
  • Short names
    DAG kinase

Gene names

    • Name
      DGKA

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Platyrrhini > Cebidae > Callitrichinae > Callithrix > Callithrix

Accessions

  • Primary accession
    U3FIG2

Subcellular Location

PTM/Processing

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain110-145EF-hand
Domain155-190EF-hand
Domain205-253Phorbol-ester/DAG-type
Domain269-319Phorbol-ester/DAG-type
Domain370-504DAGKc

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    733
  • Mass (Da)
    82,184
  • Last updated
    2022-05-25 v1
  • MD5 Checksum
    A2149DADCF89D6E8212250AE883667F4
MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDDVPSHLSLALFQSFETGHCLNETNVTKDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVEKIILQMMRVAEYLDWDVSELRPILQEMMKQIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCASGRCDRCQKKIRIYHSLTGVHCVWCHLEIHDDCLQAVGQECDCGLLRDHILPPSSIYPSVLATGSDRKNSKTSQKTMDDLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTKRPHGDICGINQALGVTAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GAMT01001721
EMBL· GenBank· DDBJ
JAB10140.1
EMBL· GenBank· DDBJ
mRNA
GAMT01001720
EMBL· GenBank· DDBJ
JAB10141.1
EMBL· GenBank· DDBJ
mRNA
GAMS01003133
EMBL· GenBank· DDBJ
JAB20003.1
EMBL· GenBank· DDBJ
mRNA
GAMR01003244
EMBL· GenBank· DDBJ
JAB30688.1
EMBL· GenBank· DDBJ
mRNA
GAMP01010419
EMBL· GenBank· DDBJ
JAB42336.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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