U3FIG2 · U3FIG2_CALJA
- ProteinDiacylglycerol kinase
- GeneDGKA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids733 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
Catalytic activity
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1,2-didecanoyl-sn-glycerol + ATP = 1,2-didecanoyl-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1,2-dihexadecanoyl-sn-glycerol + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-O-alkyl-2-acyl-sn-glycerol + ATP = 1-O-alkyl-2-acyl-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-O-hexadecyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-O-hexadecyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-O-hexadecyl-2-acetyl-sn-glycerol + ATP = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-O-hexadecyl-sn-glycerol + ATP = 1-O-hexadecyl-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 2-(9Z-octadecenoyl)-glycerol + ATP = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
Pathway
Lipid metabolism; glycerolipid metabolism.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP-dependent diacylglycerol kinase activity | |
Molecular Function | calcium ion binding | |
Biological Process | glycerolipid metabolic process | |
Biological Process | phospholipase C-activating G protein-coupled receptor signaling pathway | |
Biological Process | platelet activation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDiacylglycerol kinase
- EC number
- Short namesDAG kinase
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Platyrrhini > Cebidae > Callitrichinae > Callithrix > Callithrix
Accessions
- Primary accessionU3FIG2
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 110-145 | EF-hand | |||
Domain | 155-190 | EF-hand | |||
Domain | 205-253 | Phorbol-ester/DAG-type | |||
Domain | 269-319 | Phorbol-ester/DAG-type | |||
Domain | 370-504 | DAGKc | |||
Sequence similarities
Belongs to the eukaryotic diacylglycerol kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length733
- Mass (Da)82,184
- Last updated2022-05-25 v1
- MD5 ChecksumA2149DADCF89D6E8212250AE883667F4
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GAMT01001721 EMBL· GenBank· DDBJ | JAB10140.1 EMBL· GenBank· DDBJ | mRNA | ||
GAMT01001720 EMBL· GenBank· DDBJ | JAB10141.1 EMBL· GenBank· DDBJ | mRNA | ||
GAMS01003133 EMBL· GenBank· DDBJ | JAB20003.1 EMBL· GenBank· DDBJ | mRNA | ||
GAMR01003244 EMBL· GenBank· DDBJ | JAB30688.1 EMBL· GenBank· DDBJ | mRNA | ||
GAMP01010419 EMBL· GenBank· DDBJ | JAB42336.1 EMBL· GenBank· DDBJ | mRNA |