T0V6T3 · T0V6T3_LACLC
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids686 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 31-35 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 80-81 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 109 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 111 | N6-AMP-lysine intermediate | |||
Binding site | 132 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 166 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 280 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 304 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 430 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 433 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 448 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 453 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Lactococcus > Lactococcus cremoris subsp. cremoris
Accessions
- Primary accessionT0V6T3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Sequence
- Sequence statusComplete
- Length686
- Mass (Da)76,795
- Last updated2013-10-16 v1
- ChecksumCD234C049EDE7566
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ATBE01000351 EMBL· GenBank· DDBJ | EQC93890.1 EMBL· GenBank· DDBJ | Genomic DNA |