T0V6T3 · T0V6T3_LACLC

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site31-35NAD+ (UniProtKB | ChEBI)
Binding site80-81NAD+ (UniProtKB | ChEBI)
Binding site109NAD+ (UniProtKB | ChEBI)
Active site111N6-AMP-lysine intermediate
Binding site132NAD+ (UniProtKB | ChEBI)
Binding site166NAD+ (UniProtKB | ChEBI)
Binding site280NAD+ (UniProtKB | ChEBI)
Binding site304NAD+ (UniProtKB | ChEBI)
Binding site430Zn2+ (UniProtKB | ChEBI)
Binding site433Zn2+ (UniProtKB | ChEBI)
Binding site448Zn2+ (UniProtKB | ChEBI)
Binding site453Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      LLT3_07065

Organism names

Accessions

  • Primary accession
    T0V6T3

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain611-686BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    686
  • Mass (Da)
    76,795
  • Last updated
    2013-10-16 v1
  • Checksum
    CD234C049EDE7566
MNIESKIKEFTDQLNQFAYEYYTLDEPSVEDSEYDRLYHELVKLEQENPQLVRADSPTHRTGGVILDGFVKFRHPYNLYSLGDVFSREELALWEERVRKEIVNPEYICELKIDGLSLSLYYENGLLVTAATRGDGTTGENITENVKRIKDVPLKLKEAIDIVVRGEAYLPRQNFAKLNAERELAGAAPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELGFQVNPERKFARNLDEIWEFIEEATRLRDELPYDIDGVVIKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWTVGRTGVVTPTANMTPVLLAQTTVARATLHNVDYIQEKDIRNHDKVMIYKAGDIIPKVGKVLLEKRKPAKHLRKTHRANKYVWTRFGRRSVEKGLVFSKREDNRVKIPTQCPECDSDLIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVISQLFDKKLVTDVADLYQLTVEDLLTLDKVKETLAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALSQASEEEISEIPSLGGVIATAVVSYFETAGAKTLLDELENAGLNFAYLGAVNVEGILSGKTVVLTGKLTTLKRKEAKEKLEALGANVSGSVSKKTDLVVAGEEAGSKLTKAQDLGIEIWSEQDLLDL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ATBE01000351
EMBL· GenBank· DDBJ
EQC93890.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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