S0DZM7 · BIK1_GIBF5
- ProteinBikaverin polyketide synthase bik1
- Genebik1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2036 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Polyketide synthase; part of the gene cluster that mediates the biosynthesis of bikaverin, a red pigment also considered as a mycotoxin (PubMed:12409099, PubMed:17696354, PubMed:19400779).
The first stage is catalyzed by the polyketide synthase bik1, which catalyzes the formation of the intermediate SMA76a also knowm as pre-bikaverin (PubMed:12409099, PubMed:17696354, PubMed:19400779).
FAD-dependent monooxygenase bik2 might then be responsible for the oxidation of pre-bikaverin to oxo-pre-bikaverin which is in turn methylated by the O-methyltransferase bik3 to me-oxo-pre-bikaverin (PubMed:26382642).
A further cycle of oxydation and methylation by bik2 and bik3 leads to the final product of bikaverin, via a nor-bikaverin intermediate (PubMed:19400779, PubMed:26382642).
The first stage is catalyzed by the polyketide synthase bik1, which catalyzes the formation of the intermediate SMA76a also knowm as pre-bikaverin (PubMed:12409099, PubMed:17696354, PubMed:19400779).
FAD-dependent monooxygenase bik2 might then be responsible for the oxidation of pre-bikaverin to oxo-pre-bikaverin which is in turn methylated by the O-methyltransferase bik3 to me-oxo-pre-bikaverin (PubMed:26382642).
A further cycle of oxydation and methylation by bik2 and bik3 leads to the final product of bikaverin, via a nor-bikaverin intermediate (PubMed:19400779, PubMed:26382642).
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 541 | For beta-ketoacyl synthase activity | |||
Active site | 676 | For beta-ketoacyl synthase activity | |||
Active site | 718 | For beta-ketoacyl synthase activity | |||
Active site | 997 | For acyl/malonyl transferase activity | |||
Active site | 1325 | Proton acceptor; for dehydratase activity | |||
Active site | 1511 | Proton donor; for dehydratase activity | |||
Active site | 1857 | For thioesterase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | phenol-containing compound biosynthetic process | |
Biological Process | polyketide biosynthetic process | |
Biological Process | toxin biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBikaverin polyketide synthase bik1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium > Fusarium fujikuroi species complex
Accessions
- Primary accessionS0DZM7
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000436338 | 1-2036 | Bikaverin polyketide synthase bik1 | ||
Modified residue | 1690 | O-(pantetheine 4'-phosphoryl)serine | |||
Keywords
- PTM
Expression
Induction
Expression is repressed during the growth phase, but is induced as the growth rate decreases due to nitrogen depletion of the culture medium (PubMed:12409099).
Highly expressed only under acidic culture conditions (PubMed:12409099, PubMed:19400779).
Also induced by salt starvation (PubMed:19838698).
Expression is repressed by regulatory gene, areA, a transcriptional regulator responsible for the activation of nitrogen assimilation genes (PubMed:12409099).
Expression is also negatively regulated by vel1 (PubMed:20572938).
Highly expressed only under acidic culture conditions (PubMed:12409099, PubMed:19400779).
Also induced by salt starvation (PubMed:19838698).
Expression is repressed by regulatory gene, areA, a transcriptional regulator responsible for the activation of nitrogen assimilation genes (PubMed:12409099).
Expression is also negatively regulated by vel1 (PubMed:20572938).
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 8-242 | N-terminal acylcarrier protein transacylase domain (SAT) | |||
Domain | 370-801 | Ketosynthase family 3 (KS3) | |||
Region | 908-1209 | Acyl/malonyl transferases | |||
Region | 1293-1425 | N-terminal hotdog fold | |||
Domain | 1293-1600 | PKS/mFAS DH | |||
Region | 1295-1599 | Product template (PT) domain | |||
Region | 1452-1600 | C-terminal hotdog fold | |||
Region | 1628-1654 | Disordered | |||
Compositional bias | 1629-1654 | Polar residues | |||
Domain | 1653-1730 | Carrier | |||
Compositional bias | 1733-1753 | Polar residues | |||
Region | 1733-1758 | Disordered | |||
Domain
Multidomain protein; including a starter unit:ACP transacylase (SAT) that selects the starter unit; a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide back- bone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm (By similarity).
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,036
- Mass (Da)221,460
- Last updated2013-09-18 v1
- ChecksumFCA97D2EB05774D4
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1629-1654 | Polar residues | |||
Compositional bias | 1733-1753 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HF679027 EMBL· GenBank· DDBJ | CCT67991.1 EMBL· GenBank· DDBJ | Genomic DNA |