Q8L2R0 · Q8L2R0_STAIN
- ProteinGlyceraldehyde-3-phosphate dehydrogenase
- Genegap
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids311 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
Catalytic activity
- D-glyceraldehyde 3-phosphate + phosphate + NAD+ = (2R)-3-phospho-glyceroyl phosphate + NADH + H+
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 4-5 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 26 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 70 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 112 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 142-144 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Active site | 143 | Nucleophile | |||
Site | 170 | Activates thiol group during catalysis | |||
Binding site | 173 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 203-204 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 226 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 308 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity | |
Molecular Function | NAD binding | |
Molecular Function | NADP binding | |
Biological Process | glucose metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlyceraldehyde-3-phosphate dehydrogenase
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus > Staphylococcus intermedius group
Accessions
- Primary accessionQ8L2R0
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-143 | Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding | |||
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length311
- Mass (Da)33,401
- Last updated2002-10-01 v1
- MD5 Checksum80F187793EB2992B0E9F0C78C235C0B2
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 1 | ||||
Non-terminal residue | 311 | ||||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF495482 EMBL· GenBank· DDBJ | AAM28562.1 EMBL· GenBank· DDBJ | Genomic DNA |