Q7DBF3 · GDPPS_ECO57
- ProteinGDP-perosamine synthase
- GeneperA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids364 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the synthesis of GDP-perosamine from GDP-4-keto-6-deoxy-D-mannose and L-glutamate. Can use only L-glutamate as amino donor.
Catalytic activity
- GDP-alpha-D-perosamine + 2-oxoglutarate = GDP-4-dehydro-alpha-D-rhamnose + L-glutamate
Cofactor
Activity regulation
Divalent ions have no significant effect on activity.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.09 mM | GDP-4-keto-6-deoxy-D-mannose | |||||
0.07 mM | GDP-4-keto-6-deoxy-D-mannose | |||||
2 mM | L-glutamate | |||||
2.3 mM | L-glutamate |
kcat is 18.98 sec-1 for GDP-4-keto-6-deoxy-D-mannose. kcat is 10.41 sec-1 for L-glutamate.
pH Dependence
Optimum pH is 7.5.
Pathway
Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | GDP-4-dehydro-6-deoxy-D-mannose-4-aminotransferase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | O antigen biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGDP-perosamine synthase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ7DBF3
- Secondary accessions
Proteomes
Phenotypes & Variants
Disruption phenotype
Mutant is deficient in expression of the O157 LPS antigen. Increases adherence to cultured epithelial cells.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000430720 | 1-364 | GDP-perosamine synthase | |||
Sequence: MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAEQNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDSDNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFGSKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQYWHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVFHTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGWRGINLPSFPSLSNEQVIYICESINEFYSDK | ||||||
Modified residue | 183 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length364
- Mass (Da)41,554
- Last updated2004-07-05 v1
- ChecksumBEDF9AAC867C97CD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE005174 EMBL· GenBank· DDBJ | AAG57096.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000007 EMBL· GenBank· DDBJ | BAB36264.2 EMBL· GenBank· DDBJ | Genomic DNA |