Q7DBF3 · GDPPS_ECO57

Function

function

Catalyzes the synthesis of GDP-perosamine from GDP-4-keto-6-deoxy-D-mannose and L-glutamate. Can use only L-glutamate as amino donor.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Activity regulation

Divalent ions have no significant effect on activity.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.09 mMGDP-4-keto-6-deoxy-D-mannose
0.07 mMGDP-4-keto-6-deoxy-D-mannose
2 mML-glutamate
2.3 mML-glutamate
kcat is 18.98 sec-1 for GDP-4-keto-6-deoxy-D-mannose. kcat is 10.41 sec-1 for L-glutamate.

pH Dependence

Optimum pH is 7.5.

Pathway

Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

GO annotations

AspectTerm
Molecular FunctionGDP-4-dehydro-6-deoxy-D-mannose-4-aminotransferase activity
Molecular Functionpyridoxal phosphate binding
Biological ProcessO antigen biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    GDP-perosamine synthase
  • EC number

Gene names

    • Name
      perA
    • Synonyms
      per
      , rfbE
    • Ordered locus names
      Z3200
      , ECs2841

Organism names

  • Taxonomic identifier
  • Strains
    • O157:H7 / EDL933 / ATCC 700927 / EHEC
    • O157:H7 / Sakai / RIMD 0509952 / EHEC
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    Q7DBF3
  • Secondary accessions
    • Q7ACQ1

Proteomes

Phenotypes & Variants

Disruption phenotype

Mutant is deficient in expression of the O157 LPS antigen. Increases adherence to cultured epithelial cells.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004307201-364GDP-perosamine synthase
Modified residue183N6-(pyridoxal phosphate)lysine

Interaction

Subunit

Homodecamer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the DegT/DnrJ/EryC1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    364
  • Mass (Da)
    41,554
  • Last updated
    2004-07-05 v1
  • Checksum
    BEDF9AAC867C97CD
MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAEQNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDSDNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFGSKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQYWHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVFHTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGWRGINLPSFPSLSNEQVIYICESINEFYSDK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE005174
EMBL· GenBank· DDBJ
AAG57096.1
EMBL· GenBank· DDBJ
Genomic DNA
BA000007
EMBL· GenBank· DDBJ
BAB36264.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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