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Q5WEN4 · LEU3_SHOC1

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site76-89NAD+ (UniProtKB | ChEBI)
Binding site96substrate
Binding site106substrate
Binding site134substrate
Site141Important for catalysis
Site191Important for catalysis
Binding site223Mg2+ (UniProtKB | ChEBI)
Binding site223substrate
Binding site247Mg2+ (UniProtKB | ChEBI)
Binding site251Mg2+ (UniProtKB | ChEBI)
Binding site281-293NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • Ordered locus names
      ABC2641

Organism names

Accessions

  • Primary accession
    Q5WEN4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000836371-3643-isopropylmalate dehydrogenase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    364
  • Mass (Da)
    39,332
  • Last updated
    2004-11-23 v1
  • MD5 Checksum
    B1EA01C10E797579F05B8154DB059B2F
MEKQILLLPGDGIGPEIIAETKRVLEEIARLYHHTFQFVEGRIGGDAIDATGSPLPEETLDLAKGSDAILLGAVGGPKWDGNAPEKRPEKGLLQIRKALGLYANLRPVRVLKPLMGASTLKEEVLADVDLLIVRELTGGLYFGEPRERRQINGEDGAVDTLLYTRGEIERIVHQAFQFARTRKKRVVSVDKANVLESSRLWREIADEVAREYPDVTLSHMLVDNAAMQLIRDPRQFDVIVTENLFGDILSDEASMITGSLGLLPSASMNTSKVGLFEPVHGSAPDIAGTGAANPLATILSAAMMLQYSFGLADEAKAIEVAVDRVLAQGVRTKDIAKASENAVSTTAITNAVMEALALKSASSR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP006627
EMBL· GenBank· DDBJ
BAD65176.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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