Q5KSN5 · ECDPS_STREO
- ProteinEnt-copalyl diphosphate synthase
- Geneent-cdps
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids511 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in viguiepinol biosynthesis. Catalyzes the conversion of geranylgeranyl diphosphate (GGDP) into copalyl diphosphate (ent-CDP).
Catalytic activity
- (2E,6E,10E)-geranylgeranyl diphosphate = ent-copalyl diphosphate
Cofactor
Note: Activity is highest with Mg2+. Can also use Zn2+, Co2+, Mn2+ and Ni2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
13.7 μM | GGDP |
kcat is 0.033 sec-1.
pH Dependence
Optimum pH is 5.5.
Temperature Dependence
Optimum temperature is 35 degrees Celsius.
Pathway
Antibiotic biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 291 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 293 | a divalent metal cation (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ent-copalyl diphosphate synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | antibiotic biosynthetic process | |
Biological Process | diterpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnt-copalyl diphosphate synthase
- EC number
- Short namesEnt-CDP synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionQ5KSN5
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000431697 | 1-511 | Ent-copalyl diphosphate synthase | ||
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length511
- Mass (Da)54,768
- Last updated2005-02-15 v1
- MD5 Checksum4A1195E8D3C8FFE035D00AB0867E7EF9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB183750 EMBL· GenBank· DDBJ | BAD86797.1 EMBL· GenBank· DDBJ | Genomic DNA |