Q4W944 · ENCA_ASPFU

Function

function

Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of endocrocin, a simple anthraquinone interesting for many biotechnological applications (PubMed:22492455, PubMed:23592999).
The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) encA (PubMed:22492455).
The atrochrysone carboxyl ACP thioesterase encB then breaks the thioester bond and releases the atrochrysone carboxylic acid from encA (PubMed:22492455).
The atrochrysone carboxylic acid is then converted to endocrocin anthrone which is further oxidized into endocrocin by the anthrone oxygenase encC (PubMed:22492455).
The exact function of encD has not been identified yet, but it negatively regulates endocrocin production, likely through the modification of endocrocin itself (PubMed:22492455).

Catalytic activity

Biotechnology

Endocrocin and related anthraquinones compounds have interesting activities for medicinal uses, including anti-inflammatory activity (PubMed:20379952).

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site564For beta-ketoacyl synthase activity
Active site699For beta-ketoacyl synthase activity
Active site740For beta-ketoacyl synthase activity
Active site1341Proton acceptor; for dehydratase activity
Active site1532Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Functionfatty acid synthase activity
Biological Processendocrocin biosynthetic process
Biological Processfatty acid biosynthetic process
Biological Processsecondary metabolic process
Biological Processsecondary metabolite biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Atrochrysone carboxylic acid synthase
  • EC number
  • Short names
    ACAS
  • Alternative names
    • Endocrocin synthesis protein A
    • Non-reducing polyketide synthase encA

Gene names

    • Name
      encA
    • ORF names
      AFUA_4G00210

Organism names

Accessions

  • Primary accession
    Q4W944

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Abolishes the production of endocrocin (PubMed:22492455, PubMed:23592999).
Leads to attenuated virulence in a toll-deficient Drosophila invasive aspergillosis model (PubMed:23592999).

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00004370471-1775Atrochrysone carboxylic acid synthase
Modified residue1735O-(pantetheine 4'-phosphoryl)serine

Keywords

Expression

Tissue specificity

Endocrocin is specifically produced in conidia.

Induction

Expression is positively regulated by the transcription factors brlA and laeA (PubMed:26242966).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region29-258N-terminal acylcarrier protein transacylase domain (SAT)
Domain391-821Ketosynthase family 3 (KS3)
Region921-1241Malonyl-CoA:ACP transacylase (MAT) domain
Region1305-1626Product template (PT) domain
Region1309-1455N-terminal hotdog fold
Domain1309-1621PKS/mFAS DH
Region1472-1621C-terminal hotdog fold
Region1672-1694Disordered
Domain1698-1775Carrier

Domain

Multidomain protein; including a starter unit:ACP transacylase (SAT) that selects the starter unit; a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm (By similarity).

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,775
  • Mass (Da)
    194,076
  • Last updated
    2005-07-05 v1
  • Checksum
    26F725DCAA4E1B11
MQGPSQLALFYFANGLPPDDIQDLFQRLRSQSKTESGWTLRAFVVQATNALREEIRQLPHHLRNPLTPLDNALDLAVVPDWRRGPLAGALEGVLLCLIEIGSLIAYYERTPDPFKFSARTACFTGIGTGLLAAAAAAASPTLADLPRLGAAAVRIALRMGVLVAETSQSVEAREPDAPADNWAAVVMDLDEDTVREELNLFNASTNNLGPSRLFISAGGTDNVTISGPPSKLRQVFRVSEKLRSARYAQLPVYGGLCHAPHLYNCHHWTWIMEPINGAAFNQNMVDTAPLFSAGDDVPFEASTPRQLFESVVCDLLMGMIRWNRAVDGVVELLGQTLPSECQVYAFRPCAVVTGMVASGQVKLPHCQFQTHDLLGWTCHDDTDNGPTCREDSSIAIVGMACRFPGGANDLNQFWDLLEQGADVHRRVPADRYDVESHTDTSGKSRNTSLTPFGCFIDQPGLFDAGFFDMSPREAMQTDPMHRLALMTAYEALEQAGFVPNRTESTHLKRIGTFYGQSCDDYREANAGQEVDTYYIPGGCRAFAPGRINYFFKFSGPSFDCDTACSSSLATIQMACTSLQHGDTNMAVAGGLNILTNSDGFAGLSRGHFLSKTGGCKTFDCNADGYCRADGIGSIVLKRLDDAQRDNDHIFGIILAAATNHSARAISITHPHAPSQAELYRDILTRAGVSPLDVDFIEMHGTGTQAGDSTEMESITSVFSPGVPKRSRPLYIGSVKANVGHGEAAAGVMSLIKVLLVLQRQAIPKHVGIKTALNPRFPNLDRLNVRIPHDQVPWPRSPTRKRYALVNNFSAAGGNTSLLIEEPPVRPEPKADPRAAFTVAVSAKSKASLKNNLRSFLAYLESQPSISLAHLSYTTTARRMHHNHRIAVHGSTLSSIMQELEPYLPAVDTHRPVPNTPPSIAFVFSGQGSFYTGIARQLYEHHPGFRLQITRLHNICLSHGFPSFRRAITGDLSNDGSEAEPIITHLTIVCVSIALCRLWETLGVKPCVVAGASLGEFAALYAAGVLSASDAIYLVGRRAQLLQELCTPNTHAMLAVRATVEQIRNVLAGQPYEVACINGSSDITLSCSVADIINLQLAIEQHGYKCTRLDVPFAFHSAQMDPLLGPFEHIARGVTFKAPNIPVMSPSLGDCVFDGKTINASYMCNVTRNPVKFVDALETARGMDLVDAKTVWVEIGPHASYSRFVGSAMPPGTATIASLNRNEDNWSTFARSMAQLHNLGVDLNWHEWHAPFESELRLLTDLPAYQWNMKNYWIQYNGDWMLRKDGKSSAAAASHPHQAIPPALRTSLVHRLVCESVQETRVEVIVESDILHPDFFEAMNGHRMNGCAVATTAIHADIAFTLAKYLYSSIMPNSTDAPAINVKNMQVQHGLVARKDRSRPQLIRIRGIADVTRGLVSLSWHLVDEQGRRVEESFATAVAEFGNHEAWLEEWSPMTHLVVSRIDVLQRLADDGTANRLSRDMVYMLFNNLVDYAEKYRGMQMVVLHGLEAMANVTLAAPEQSGGKWTVAPHYIDSVVHLAGFILNGGNGLDPRRNFYVTPGWKSMRFARPLVPGVRYQSYVKMMPIREQSGFYAGDVYILHEGQIVGLVGGITFRTFPRSLINTFFSPPDTMTHGGSQAGSVHQPACSERTLPVGPARQDSAARETLCQGHGLSRTVMDSSDSSPATTLTPPTLPSVAASTESPIVHRAMALIAAETAIELTELSDETAFSSIGVDSLLSLVLAEKFTAEFHLDFRSSLFLDCPTIGDLKAWLIDYC

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHF01000017
EMBL· GenBank· DDBJ
EAL84397.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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