P0DXA6 · CDNE_STASC

  • Protein
    Cyclic GMP-AMP synthase CdnE03
  • Gene
    cdnE
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Cyclic nucleotide synthase (second messenger synthase) of a CBASS antivirus system (PubMed:37968393).
CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage (PubMed:37968393).
The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals (PubMed:37968393).
The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection (PubMed:37968393).
The effector for this system is downstream Cap15 (PubMed:37968393).
A type I-B CBASS system (PubMed:32839535).
Cyclic dinucleotide synthase that catalyzes the synthesis of 3',2'-cyclic GMP-AMP (cGAMP) from GTP and ATP upon activation by viral-derived cabRNA (PubMed:37968393).
Binds cabRNA via positive charges in its N-terminus (PubMed:37968393).
Protects S.aureus against phage infection. When the CBASS operon (cdnE-cap15) is introduced in S.aureus strain RN4220 there is strong protection against lytic DNA phages 80alpha-vir and phi-NM1-gamma-6 but little to no protection against phages phi-NM4-gamma-4 or phi-12-gamma-3 (PubMed:37968393).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Activity regulation

Activated by a virus-derived, approximately 400 nucleotide RNA (called CBASS-activating bacteriophage RNA, cabRNA) that begins in the viral terminase subunit terS and extends into terL (PubMed:37968393).
RNA secondary and/or tertiary structure, as well as viral infection itself, are important for CdnE activation (PubMed:37968393).
A much longer RNA (escaper RNA) with a different secondary structure, derived from a terS-mutated virus still binds to this protein, but does not activate its nucleotide cyclase activity (PubMed:37968393).
Shorter viral-derived RNAs (34 and 49 nt) with extensive predicted secondary structure also activate the enzyme, although not as well as full-length cabRNA (PubMed:37968393).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site87Mg2+ (UniProtKB | ChEBI)
Binding site89ATP (UniProtKB | ChEBI)
Binding site89Mg2+ (UniProtKB | ChEBI)
Binding site144-145ATP (UniProtKB | ChEBI)
Binding site159ATP (UniProtKB | ChEBI)
Binding site159Mg2+ (UniProtKB | ChEBI)
Binding site224GTP (UniProtKB | ChEBI)
Binding site243GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular FunctionRNA binding
Biological Processdefense response to virus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cyclic GMP-AMP synthase CdnE03
  • EC number
  • Short names
    Ssc-CdnE03
  • Alternative names
    • cGAS/DncV-like nucleotidyltransferase
      (CD-NTase057
      )

Gene names

    • Name
      cdnE
    • ORF names
      OA96_00210

Organism names

  • Taxonomic identifier
  • Strain
    • 2142-05
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus

Accessions

  • Primary accession
    P0DXA6

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis9Does not make cyclic 3',2'-cGAMP (3',2'-cGAMP), does not bind cabRNA, does not protect against phage.
Mutagenesis13Substantially reduces synthesis of 3',2'-cGAMP, weakly binds cabRNA, reduced protection against phage.
Mutagenesis87-89Does not protect against phage, does not make 3',2'-cGAMP.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004598311-324Cyclic GMP-AMP synthase CdnE03

Structure

3D structure databases

Family & Domains

Sequence similarities

Belongs to the CD-NTase family. E03 subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    324
  • Mass (Da)
    38,298
  • Last updated
    2024-03-27 v1
  • Checksum
    B96161166556485F
MLFTEEQLKLYSKPLSESEKEKCENAIRIIQESLESLGYETKKGIHRNNEDTLSYQIKMTNPSKDYELSIFVKGSYATNTNVRQNSDVDIAVVKESEFFDKYREGKTRENYKFVSSNKPPYHFKDEVEEALIERFGRSEVRRGNKAIRINGNTYRKETDCVPCFRYRDYSNDYMDDPNNFIGGITIYSDKGERIINYPEQHINNSVIKNNNTNYKYKKMVRIIKEIRYQLIDSKNENAKQTSSFGVEGLFWNIPDYKYSNDEMLGDTFNALIAFLIDNIDKLREFKEPNDILNLCDSQEKNNVYKNFILDVKNYFEYSGEKKYE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009762
EMBL· GenBank· DDBJ
AKS70342.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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