P0DL27 · VSP41_BOTPI
- ProteinThrombin-like enzyme BpirSP41
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Snake venom serine protease that interferes with the hemostatic system of the prey. It almost completely degrades both Aalpha (FGA) and Bbeta (FGB) chains of fibrinogen. It presents a higher ability to degrade fibrin clots than BpirSP27. It hydrolyzes chromogenic substrates S-2238 (used for testing thrombin activity), S-2222 (factor Xa), S-2266 (glandular kallikrein and factor XIa), and S-2302 (plasma kallikrein, factor XIa and XIIa). It shows a decrease in the clotting time of human plasma in the presence of increasing doses of the enzyme. Its minimum coagulant dose (MCD) is 20 ug. It promotes platelet aggregation with a maximum of aggregation of 20%, regardless of the concentration increase or the presence of calcium. It also shows 40% inhibition of the hemolytic activity promoted by the complement pathways and possess only a minor role in the induction of edema and pain in rat.
Miscellaneous
Acidic enzyme (pI is 3.7).
Negative results: does not degrade the gamma chain of fibrinogen (FGG). It has no activity on S-2251 (used for testing plasmin activity) (PubMed:22819993).
Activity regulation
Inhibited by serine protease inhibitors PMSF, benzamidine, leupeptin and aprotinin, as well as by copper ions (Cu2+). Not inhibited by metalloprotease inhibitors EDTA, EGTA and 1,10-phenanthroline, as well as by barium (Ba2+) and calcium ion (Ca2+).
pH Dependence
Optimum pH is 6.0-10.5.
Temperature Dependence
Optimum temperature is 4-60 degrees Celsius.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 40 | Charge relay system | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular space | |
Molecular Function | serine-type endopeptidase activity | |
Molecular Function | toxin activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThrombin-like enzyme BpirSP41
- EC number
- Short namesSVTLE
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Viperidae > Crotalinae > Bothrops
Accessions
- Primary accessionP0DL27
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000422277 | 1-50 | Thrombin-like enzyme BpirSP41 | ||
Disulfide bond | 7↔? | ||||
Disulfide bond | 25↔41 | ||||
Post-translational modification
N-glycosylated.
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-50 | Peptidase S1 | |||
Sequence similarities
Belongs to the peptidase S1 family. Snake venom subfamily.
Family and domain databases
Sequence
- Sequence statusFragment
- Length50
- Mass (Da)5,836
- Last updated2013-05-01 v1
- MD5 Checksum120FCAA3BDBB4DACA144DAFC205702EF
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 50 | ||||
Mass Spectrometry
Keywords
- Technical term