O87876 · BCRA_THAAR
- ProteinBenzoyl-CoA reductase subunit A
- GenebcrA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids438 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the anaerobic reduction of benzoyl-CoA and 3-hydroxybenzoyl-CoA to form cyclohexa-1,5-diene-1-carbonyl-CoA and 3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA, respectively. The enzyme also reduces other benzoyl-CoA analogs with small substituents at the aromatic ring.
Catalytic activity
- cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized 2[4Fe-4S]-[ferredoxin] + 2 ADP + 2 phosphate = reduced 2[4Fe-4S]-[ferredoxin] + benzoyl-CoA + 2 ATP + 2 H2O
CHEBI:57374 + RHEA-COMP:10004 CHEBI:33722 Position: 1CHEBI:33722 Position: 2+ 2 CHEBI:456216 + 2 CHEBI:43474 = RHEA-COMP:10002 CHEBI:33723 Position: 1CHEBI:33723 Position: 2+ CHEBI:57369 + 2 CHEBI:30616 + 2 CHEBI:15377
Cofactor
Note: The iron-sulfur cluster may be a [4Fe-4S] cluster.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
15 μM | benzoyl-CoA | |||||
20 μM | 3-hydroxybenzoyl-CoA | |||||
600 μM | ATP |
pH Dependence
Optimum pH is 7.2-7.5.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 298 | [4Fe-4S] cluster (UniProtKB | ChEBI); ligand shared with BcrD | |||
Binding site | 337 | [4Fe-4S] cluster (UniProtKB | ChEBI); ligand shared with BcrD | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ATP binding | |
Molecular Function | benzoyl-CoA reductase activity | |
Molecular Function | metal ion binding | |
Biological Process | catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBenzoyl-CoA reductase subunit A
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Rhodocyclales > Zoogloeaceae > Thauera
Accessions
- Primary accessionO87876
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000350730 | 1-438 | Benzoyl-CoA reductase subunit A | ||
Interaction
Subunit
Heterotetramer composed of A, B, C, and D subunits.
Structure
Sequence
- Sequence statusComplete
- Length438
- Mass (Da)48,359
- Last updated1998-11-01 v1
- Checksum8F9E470FF688EAB7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ224959 EMBL· GenBank· DDBJ | CAA12249.1 EMBL· GenBank· DDBJ | Genomic DNA |