N1MM98 · N1MM98_9SPHN

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site396Mg2+ (UniProtKB | ChEBI); catalytic
Binding site439Mg2+ (UniProtKB | ChEBI); catalytic
Binding site497Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site500Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      EBBID32_9450

Organism names

  • Taxonomic identifier
  • Strain
    • BiD32
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingobium

Accessions

  • Primary accession
    N1MM98

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain38-212S1 motif
Region124-169Disordered
Compositional bias130-144Acidic residues
Region497-500Required for zinc-mediated homotetramerization and catalytic activity
Region574-783Disordered
Compositional bias595-621Acidic residues
Compositional bias622-642Basic and acidic residues
Compositional bias643-654Basic residues
Compositional bias655-664Basic and acidic residues
Compositional bias665-691Acidic residues
Compositional bias707-721Basic residues
Region798-851Disordered
Region873-902Disordered

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    902
  • Mass (Da)
    99,691
  • Last updated
    2013-06-26 v1
  • MD5 Checksum
    60B25ACD434186AE261CEAF69352E413
MTMRMLIDARHREETRVAVVKGNRIEEFDFESAEHKQLKGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQIPREDREALLREERAHAEEEAALRADFDDEDDEDGHGSVETVEATHHHDDEDEQEAGEQAEGSETEGNGGRGNGRGRGRGRGGDDAADELRRKRMALRRRYKIQDVIKRRQVLLVQVVKEERGNKGAALTSYLSLAGRYCVLMPNTSHGGGISRKISNAADRKRLKTIIADMALPSSMGCIVRTAGLQRTKVEIKRDFDYLARLWDEIRENTMKSAAPALIHNDSDLIKRAIRDIYNKDIEEVIVEGEHGYKAAKDFMRLLMPSHARKVKGYADAVSLFQRAGVEDQLAAMYNPVVQLKSGGYLVINPTEALVSIDINSGRSTREHGIEQTAVATNLEAAREIARQLRLRDMAGLVVIDFIDMEVNSNIRKVEKAMKDALKDDRARIQVGRISGFGLMEMSRQRLRTGVLEASTRQCPHCEGTGLVRTASSAGLSALRMLEEEAARGRGNVITLRASQEAAFYVLNNKRRELDEIEQRYGVSIVVLPDGEVEGARMSVEPSGPRPERVVTYAPMDQDDDELDLIEEEEEEEEPQAEDEEEVVAEREPRGDREQGDRGEDRDGNRRRRRRRRRRGGQRDDNRVEGEAGDDSEAGAEAADAEEGATEDGEAEAEVTVTTAETEEEGAARRRGRRGRRGGRRRREGGEGTSEAGTEAVEAEPVADEAPVIEEAAVEEAPAEEAPKTRRRPRARKPKADSAAVTETVVEEAPVEVVAEAPAEVAAEEAPAKPRRGRRTKAQIEADAAAAAVAEAAPVAEEAPAKPKRTRRKKADVAVEAEAAPAVEAPAVVEEAPAAETVAAEGETEGVNEDGTPRRGWWQRTFGQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias130-144Acidic residues
Compositional bias595-621Acidic residues
Compositional bias622-642Basic and acidic residues
Compositional bias643-654Basic residues
Compositional bias655-664Basic and acidic residues
Compositional bias665-691Acidic residues
Compositional bias707-721Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAVK010000045
EMBL· GenBank· DDBJ
CCW16608.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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