F6QXW0 · DNA2_XENTR
- ProteinDNA replication ATP-dependent helicase/nuclease DNA2
- Genedna2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1048 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function (By similarity).
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 142 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 395 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 398 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 404 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 649-656 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 5'-3' DNA helicase activity | |
Molecular Function | 5'-flap endonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | nuclease activity | |
Molecular Function | single-stranded DNA helicase activity | |
Biological Process | base-excision repair | |
Biological Process | DNA double-strand break processing | |
Biological Process | DNA replication | |
Biological Process | DNA replication checkpoint signaling | |
Biological Process | DNA replication, Okazaki fragment processing |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDNA replication ATP-dependent helicase/nuclease DNA2
- Alternative names
Including 2 domains:
- Recommended nameDNA replication nuclease DNA2
- EC number
- Recommended nameDNA replication ATP-dependent helicase DNA2
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Silurana
Accessions
- Primary accessionF6QXW0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000419467 | 1-1048 | DNA replication ATP-dependent helicase/nuclease DNA2 | ||
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 86-521 | Nuclease activity | |||
Region | 522-1048 | Helicase activity | |||
Region | 683-724 | Disordered | |||
Compositional bias | 686-700 | Basic residues | |||
Compositional bias | 701-721 | Polar residues | |||
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,048
- Mass (Da)118,558
- Last updated2011-07-27 v1
- MD5 Checksum5B410277ACDA2B287741B34671B44E76
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8J0QRW9 | A0A8J0QRW9_XENTR | dna2 | 1053 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 686-700 | Basic residues | |||
Compositional bias | 701-721 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAMC01051984 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AAMC01051985 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AAMC01051986 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |