D7Y2H4 · CAP6_ECOM1
- ProteinCD-NTase-associated protein 6
- Genecap6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids311 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulates complex assembly in a CBASS antivirus system. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system (PubMed:32839535).
Binds and disassembles an active CdnC:Cap7 (Cap7 is also called HORMA) complex, inhibiting the complex's ability to synthesize cyclic nucleotide second messengers (PubMed:31932165).
An AAA+-ATPase remodeler, in the absence of foreign threat Cap6 (also called Trip13) probably maintains the Cap7 protein in its open, inactive state. Once activated (presumably by a bacteriophage protein) Cap7 binds to and activates its cognate CD-NTase (CdnC in this bacteria) to synthesize cAAA, a cyclic nucleotide second messenger. cAAA activates the NucC endonuclease which degrades all DNA in the infected cell, causing cell death and abortive phage infection (Probable)
An AAA+-ATPase remodeler, in the absence of foreign threat Cap6 (also called Trip13) probably maintains the Cap7 protein in its open, inactive state. Once activated (presumably by a bacteriophage protein) Cap7 binds to and activates its cognate CD-NTase (CdnC in this bacteria) to synthesize cAAA, a cyclic nucleotide second messenger. cAAA activates the NucC endonuclease which degrades all DNA in the infected cell, causing cell death and abortive phage infection (Probable)
Protects E.coli strain JP313 against bacteriophage lambda cI- infection. When the cdnC-cap7-cap6-nucC operon is transformed into a susceptible E.coli strain it confers bacteriophage lambda cI- immunity. Mutations in the sensor (Cap7 also called HORMA) or effector proteins (CdnC, NucC) but not the disassembly protein (Cap6 also called Trip13) no longer confer immunity. The presence of the intact operon leads to culture collapse and cell death, which occurs before the phage has finished its replication cycle, thus protecting non-infected bacteria by aborting the phage infection and preventing its propagation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 84-89 | ATP (UniProtKB | ChEBI) | |||
Binding site | 215-216 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | microtubule cytoskeleton | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Biological Process | defense response to virus |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCD-NTase-associated protein 6
- Short namesCap6
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionD7Y2H4
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 87 | Partially inhibits second messenger synthesis by CdnC:Cap7:DNA complex. | |||
Mutagenesis | 159 | Stabilizes a 1:1:6 CdnC:Cap7:Cap6 complex, probably prevents ATP hydrolysis. Still confers phage immunity. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000451843 | 1-311 | CD-NTase-associated protein 6 | ||
Interaction
Subunit
Homohexamer. Forms a 1:1:6 CdnC:Cap7:Cap6 complex.
Structure
Family & Domains
Domain
In the disassembly complex (PDB:6P8V) Cap6 (this protein) crystallizes as a right-handed spiral; the top 4 subunits bind ATP while the bottom 2 do not. A CdnC monomer lies along the surface of the hexamer at the interface of subunits 5 and 6, with Cap7 (also called HORMA) at its tip, over the central hexamer pore. The N-terminus of Cap7 extends into the pore, contacting 5/6 Cap6 subunits.
Sequence similarities
Belongs to the AAA ATPase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length311
- Mass (Da)34,358
- Last updated2010-10-05 v1
- Checksum745DAF9F1763971B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ADTL01000141 EMBL· GenBank· DDBJ | EFJ98158.1 EMBL· GenBank· DDBJ | Genomic DNA |