D5U4G0 · D5U4G0_BRAM5
- ProteinGlutamine-dependent NAD(+) synthetase
- GenenadE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids630 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.
Catalytic activity
- deamido-NAD+ + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD+ + H+
Pathway
Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 41 | Proton acceptor; for glutaminase activity | |||
Active site | 123 | For glutaminase activity | |||
Binding site | 129 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 175 | Nucleophile; for glutaminase activity | |||
Binding site | 206 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 212 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 323-330 | ATP (UniProtKB | ChEBI) | |||
Binding site | 414 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
Binding site | 444 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
Binding site | 587 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | glutaminase activity | |
Molecular Function | NAD+ synthase (glutamine-hydrolyzing) activity | |
Molecular Function | NAD+ synthase activity | |
Biological Process | NAD biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamine-dependent NAD(+) synthetase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Spirochaetota > Spirochaetia > Brachyspirales > Brachyspiraceae > Brachyspira
Accessions
- Primary accessionD5U4G0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-280 | CN hydrolase | |||
Sequence similarities
Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length630
- Mass (Da)71,160
- Last updated2010-07-13 v1
- MD5 ChecksumE536C7003EDC67B912DCA869DED57ECE
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001959 EMBL· GenBank· DDBJ | ADG70205.1 EMBL· GenBank· DDBJ | Genomic DNA |