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D5U4G0 · D5U4G0_BRAM5

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site41Proton acceptor; for glutaminase activity
Active site123For glutaminase activity
Binding site129L-glutamine (UniProtKB | ChEBI)
Active site175Nucleophile; for glutaminase activity
Binding site206L-glutamine (UniProtKB | ChEBI)
Binding site212L-glutamine (UniProtKB | ChEBI)
Binding site323-330ATP (UniProtKB | ChEBI)
Binding site414deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site444deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site587deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • Ordered locus names
      Bmur_0095

Organism names

Accessions

  • Primary accession
    D5U4G0

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-280CN hydrolase

Sequence similarities

Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    630
  • Mass (Da)
    71,160
  • Last updated
    2010-07-13 v1
  • MD5 Checksum
    E536C7003EDC67B912DCA869DED57ECE
MKIAISQIEIIPSMPCDNTVRIISAISSAKKNNADIIVFPELCVSGYMLGDMWESKGFINECEELGEEIIKSSNGIYVIFGNVASDKSKKNFDGRLRKYNALFAAKDGKLIHNNTTPYPFIIKSLLPNYKEFEDPRHFFSLKDLAFENNADIKEYLKPLEIECNGEKIKLGLTICEDAWSSNYSFSPMDIININKDVDLFINISSSPYTLVKDTKRHKMYGEIASKHNIPLVYVNNVGIQNNGKTVYTFDGGSSVYDNNGNLLLTANRYEDNVYYIEMDIKNKSYPKPIEIQEDDEYKFIYDTVIYGIKKFTKSIGVKKIVIGVSGGIDSALSSAMYVSALGKDNVYLVNMPSKFNSSTTKDLAKTLADNLGCSYMVVPIQESVDYTVKQLESSPIIKDGNENYLKLSSFVIENIQARDRSSRILSAIAASIGGVFTCNANKTETMVGYSTMYGDSAGFFACLADLWKYQIYGLAKYVNEKVFGEEIIPEGTINIVPSAELSTAQAVDEGKGDPIKYDYHDYLFKFLMESWNRIILEDVLELYINDKLEEAIGCQKGILNKYFTTAHDFVDDLEKWWKQFMGMGITKRIQAPPILAVSRRSFGYGNREAQNRVYYTARYLYLKNKVLSLD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001959
EMBL· GenBank· DDBJ
ADG70205.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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