D0LDT3 · PAFA_GORB4
- ProteinPup--protein ligase
- GenepafA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids452 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Miscellaneous
The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.
Catalytic activity
Pathway
Protein degradation; proteasomal Pup-dependent pathway.
Protein modification; protein pupylation.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 9 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 53 | ATP (UniProtKB | ChEBI) | |||
Binding site | 55 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 57 | Proton acceptor | |||
Binding site | 63 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 66 | ATP (UniProtKB | ChEBI) | |||
Binding site | 419 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ligase activity, forming carbon-nitrogen bonds | |
Molecular Function | magnesium ion binding | |
Molecular Function | ubiquitin-like protein transferase activity | |
Biological Process | modification-dependent protein catabolic process | |
Biological Process | proteasomal protein catabolic process | |
Biological Process | protein pupylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePup--protein ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Gordoniaceae > Gordonia
Accessions
- Primary accessionD0LDT3
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000395917 | 1-452 | Pup--protein ligase | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length452
- Mass (Da)51,086
- Last updated2010-08-10 v2
- ChecksumB6AA1B7E82250549
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001802 EMBL· GenBank· DDBJ | ACY21706.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |