D0LDT3 · PAFA_GORB4

Function

function

Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.

Miscellaneous

The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.

Catalytic activity

  • ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine.
    EC:6.3.1.19 (UniProtKB | ENZYME | Rhea)

Pathway

Protein degradation; proteasomal Pup-dependent pathway.
Protein modification; protein pupylation.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site9Mg2+ (UniProtKB | ChEBI)
Binding site53ATP (UniProtKB | ChEBI)
Binding site55Mg2+ (UniProtKB | ChEBI)
Active site57Proton acceptor
Binding site63Mg2+ (UniProtKB | ChEBI)
Binding site66ATP (UniProtKB | ChEBI)
Binding site419ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionligase activity, forming carbon-nitrogen bonds
Molecular Functionmagnesium ion binding
Molecular Functionubiquitin-like protein transferase activity
Biological Processmodification-dependent protein catabolic process
Biological Processproteasomal protein catabolic process
Biological Processprotein pupylation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Pup--protein ligase
  • EC number
  • Alternative names
    • Proteasome accessory factor A
    • Pup-conjugating enzyme

Gene names

    • Name
      pafA
    • Ordered locus names
      Gbro_2465

Organism names

Accessions

  • Primary accession
    D0LDT3

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003959171-452Pup--protein ligase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    452
  • Mass (Da)
    51,086
  • Last updated
    2010-08-10 v2
  • Checksum
    B6AA1B7E82250549
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLQNGARLYLDVGSHPEYATAECDSLIQLINHDRAGELVLEDLLVDAEQRLSDEGIGGDIYLFKNNTDSAGNSYGCHENYLVVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSEMTTLLKVGSAALVLEMIEAGVVFRDFALDNPIRAIREASHDPTGRRPVRLAGGRQASALDIQREYHARAVEHMTNRRPDPEMDMVVDLWGRMLDAVERDDFSKVDTEVDWVIKRKLFQRYQDKYSMELSDPKIAQLDLAFHDIKRGRGVFDVLSRKGLVTRATTDEAIATAVNEPPQTTRAKLRGDFISAAQKAGRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVDRLIASM

Sequence caution

The sequence ACY21706.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001802
EMBL· GenBank· DDBJ
ACY21706.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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