B7JWW6 · MOAA_RIPO1
- ProteinGTP 3',8-cyclase
- GenemoaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids329 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
Catalytic activity
- GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H+
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 8 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 15 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 19 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 21 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 22 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 60 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 64 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 91 | GTP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 115 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 155 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 189 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 252 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C | ||||||
Binding site | 255 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C | ||||||
Binding site | 257-259 | GTP (UniProtKB | ChEBI) | ||||
Sequence: RMR | ||||||
Binding site | 269 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cyclic pyranopterin monophosphate synthase activity | |
Molecular Function | GTP 3',8'-cyclase activity | |
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTP 3',8-cyclase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Oscillatoriophycideae > Chroococcales > Aphanothecaceae > Rippkaea > Rippkaea orientalis
Accessions
- Primary accessionB7JWW6
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000139317 | 1-329 | GTP 3',8-cyclase | |||
Sequence: MNPVDYLRISLIDRCNFRCQYCMPEGVKLDYILRSELLTDDELLTLIKAVFIPLGFTKFRLTGGEPLLRPGVVQLVRDIAALPQTEDLSMTTNGFLLSRMAKELYQAGLKRINISLDSLNQETFDKIIGNFGASKWQQTWEGIQTAYEVGFNPLKLNVVIIPGINEGEIEDLAALTIDRNWHVRFIEFMPIGNRELFSDRAWVPSEEIRQTIRQKWGLIESTIKGNGPADVFQIPGGKGTLGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQIDLKTALRQGIKTTELREQVREILAIKPNINYQERESGTQTGTYQRTMSQIGG |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-229 | Radical SAM core | ||||
Sequence: MNPVDYLRISLIDRCNFRCQYCMPEGVKLDYILRSELLTDDELLTLIKAVFIPLGFTKFRLTGGEPLLRPGVVQLVRDIAALPQTEDLSMTTNGFLLSRMAKELYQAGLKRINISLDSLNQETFDKIIGNFGASKWQQTWEGIQTAYEVGFNPLKLNVVIIPGINEGEIEDLAALTIDRNWHVRFIEFMPIGNRELFSDRAWVPSEEIRQTIRQKWGLIESTIKGNGPA |
Sequence similarities
Belongs to the radical SAM superfamily. MoaA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length329
- Mass (Da)37,343
- Last updated2009-02-10 v1
- Checksum677E1CCAC078E3E7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001287 EMBL· GenBank· DDBJ | ACK65815.1 EMBL· GenBank· DDBJ | Genomic DNA |