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B5L3X1 · WFED_SHIBO

  • Protein
    UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase
  • Gene
    wfeD
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Galactosyltransferase that adds one galactose residue in the beta-1-4 linkage to GlcNAc-alpha-pyrophosphate-lipid in the biosynthesis of the O-polysaccharide repeating unit of the O antigen.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Pb2+ (UniProtKB | Rhea| CHEBI:49807 )

Note: Divalent metal cation. Mn2+, Ni2+, and Pb2+ enhance the enzyme activity.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.25 mMUDP-alpha-D-galactose
0.1 mMN-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
40 μmol/h/mgwith UDP-alpha-D-galactose as substrate
42 μmol/h/mgwith N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol as substrate

Pathway

Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site101Nucleophile

GO annotations

AspectTerm
Molecular Functionbeta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
Molecular Functionhexosyltransferase activity
Biological ProcessO antigen biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase
  • EC number

Gene names

    • Name
      wfeD

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Shigella

Accessions

  • Primary accession
    B5L3X1

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis99Does not affect catalytic activity.
Mutagenesis101Abolishes catalytic activity.
Mutagenesis103Does not affect catalytic activity.
Mutagenesis210Does not affect catalytic activity.
Mutagenesis211Abolishes catalytic activity.
Mutagenesis212Does not affect catalytic activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004241321-237UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyltransferase 26 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    237
  • Mass (Da)
    27,941
  • Last updated
    2008-10-14 v1
  • MD5 Checksum
    B8EE1473CBED113320EACBE4116E9E21
MIDNLIKRTPEINRLLENKRVTGVVTFVNPYSYYKIKEYNKISQLDYIYIDGILLLKLFNFVNGTKIKRHSFDYSSIAKTVFNYSIQNKMKIGLIGSKDYEIEQAVKNIRKKHPGIDISYFHSGYFSSLEEKSSVIDSVIKKSDIIICGLGTPAQEELALDIKIKSNEHLIFTCGGFFTQTASRADFYYPWIKRYNLMWLQRIVLYKHVRKRFFIDYPKFIVRFISENLMKIFTRSN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU296409
EMBL· GenBank· DDBJ
ACD37055.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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