B5L3X1 · WFED_SHIBO
- ProteinUDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase
- GenewfeD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids237 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Galactosyltransferase that adds one galactose residue in the beta-1-4 linkage to GlcNAc-alpha-pyrophosphate-lipid in the biosynthesis of the O-polysaccharide repeating unit of the O antigen.
Catalytic activity
- N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl diphosphate + UDP-alpha-D-galactose = beta-D-Gal-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl diphosphate + UDP + H+
Cofactor
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )
Pb2+ (UniProtKB | Rhea| CHEBI:49807 )
Note: Divalent metal cation. Mn2+, Ni2+, and Pb2+ enhance the enzyme activity.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.25 mM | UDP-alpha-D-galactose | |||||
0.1 mM | N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
40 μmol/h/mg | with UDP-alpha-D-galactose as substrate | ||||
42 μmol/h/mg | with N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol as substrate |
Pathway
Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 101 | Nucleophile | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity | |
Molecular Function | hexosyltransferase activity | |
Biological Process | O antigen biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameUDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Shigella
Accessions
- Primary accessionB5L3X1
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 99 | Does not affect catalytic activity. | |||
Mutagenesis | 101 | Abolishes catalytic activity. | |||
Mutagenesis | 103 | Does not affect catalytic activity. | |||
Mutagenesis | 210 | Does not affect catalytic activity. | |||
Mutagenesis | 211 | Abolishes catalytic activity. | |||
Mutagenesis | 212 | Does not affect catalytic activity. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000424132 | 1-237 | UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase | ||
Structure
Sequence
- Sequence statusComplete
- Length237
- Mass (Da)27,941
- Last updated2008-10-14 v1
- MD5 ChecksumB8EE1473CBED113320EACBE4116E9E21
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU296409 EMBL· GenBank· DDBJ | ACD37055.1 EMBL· GenBank· DDBJ | Genomic DNA |