A9A5K8 · BIOD_NITMS
- ProteinATP-dependent dethiobiotin synthetase BioD
- GenebioD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids228 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity
- (7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H+
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-17 | ATP (UniProtKB | ChEBI) | |||
Binding site | 16 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 37 | ||||
Binding site | 53 | ATP (UniProtKB | ChEBI) | |||
Binding site | 53 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 114 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 114-117 | ATP (UniProtKB | ChEBI) | |||
Binding site | 174-175 | ATP (UniProtKB | ChEBI) | |||
Binding site | 203-205 | ATP (UniProtKB | ChEBI) | |||
Binding site | 210 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | dethiobiotin synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent dethiobiotin synthetase BioD
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Nitrososphaerota > Nitrososphaeria > Nitrosopumilales > Nitrosopumilaceae > Nitrosopumilus
Accessions
- Primary accessionA9A5K8
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000133217 | 1-228 | ATP-dependent dethiobiotin synthetase BioD | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length228
- Mass (Da)25,223
- Last updated2008-01-15 v1
- MD5 Checksum7F335CCBB8DACC40322A66BEC24E6918
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000866 EMBL· GenBank· DDBJ | ABX12719.1 EMBL· GenBank· DDBJ | Genomic DNA |