A5F120 · NHAD_VIBC3
- ProteinNa(+)/H(+) antiporter NhaD
- GenenhaD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids477 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Na+/H+ antiporter that extrudes sodium in exchange for external protons. Can also transport lithium.
pH Dependence
Optimum pH is 8.0. Totally inactive at pH 9.0.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | antiporter activity | |
Biological Process | sodium ion transport |
Keywords
- Biological process
- Ligand
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNa(+)/H(+) antiporter NhaD
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio
Accessions
- Primary accessionA5F120
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-33 | Periplasmic | |||
Transmembrane | 34-51 | Helical | |||
Topological domain | 52-60 | Cytoplasmic | |||
Transmembrane | 61-78 | Helical | |||
Topological domain | 79-94 | Periplasmic | |||
Transmembrane | 95-112 | Helical | |||
Topological domain | 113-132 | Cytoplasmic | |||
Transmembrane | 133-150 | Helical | |||
Topological domain | 151-154 | Periplasmic | |||
Transmembrane | 155-171 | Helical | |||
Topological domain | 172-181 | Cytoplasmic | |||
Transmembrane | 182-206 | Helical | |||
Topological domain | 207-220 | Periplasmic | |||
Transmembrane | 221-238 | Helical | |||
Topological domain | 239-258 | Cytoplasmic | |||
Transmembrane | 259-277 | Helical | |||
Topological domain | 278-281 | Periplasmic | |||
Transmembrane | 282-299 | Helical | |||
Topological domain | 300-344 | Cytoplasmic | |||
Transmembrane | 345-362 | Helical | |||
Topological domain | 363-378 | Periplasmic | |||
Transmembrane | 379-403 | Helical | |||
Topological domain | 404-413 | Cytoplasmic | |||
Transmembrane | 414-438 | Helical | |||
Topological domain | 439-454 | Periplasmic | |||
Transmembrane | 455-472 | Helical | |||
Topological domain | 473-477 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 93 | Shifts the pH optimum to more acidic pH. | |||
Mutagenesis | 100 | No change in activity. Shifts the pH optimum to more alkaline pH. | |||
Mutagenesis | 150 | Lack of activity. | |||
Mutagenesis | 154 | Lack of activity. | |||
Mutagenesis | 155 | Lack of activity. | |||
Mutagenesis | 157 | Decrease in activity. | |||
Mutagenesis | 189 | Lack of activity. | |||
Mutagenesis | 199 | Lack of activity. | |||
Mutagenesis | 201 | Lack of activity. | |||
Mutagenesis | 202 | Lack of activity. | |||
Mutagenesis | 210 | Shifts the pH optimum to more acidic pH. | |||
Mutagenesis | 251 | No change in activity. Shifts the pH optimum to more alkaline pH. | |||
Mutagenesis | 274 | No change in activity. | |||
Mutagenesis | 278 | No change in activity. | |||
Mutagenesis | 342 | No change in activity. Shifts the pH optimum to more alkaline pH. | |||
Mutagenesis | 344 | Lack of activity. | |||
Mutagenesis | 344 | Decrease in activity. | |||
Mutagenesis | 345 | Lack of activity. | |||
Mutagenesis | 389 | Lack of activity. | |||
Mutagenesis | 390 | No change in activity. | |||
Mutagenesis | 393 | No change in activity. Shifts the pH optimum to more alkaline pH. | |||
Mutagenesis | 393 | Lack of activity. | |||
Mutagenesis | 394 | Lack of activity. | |||
Mutagenesis | 425 | Lack of activity. | |||
Mutagenesis | 428 | Decrease in activity. | |||
Mutagenesis | 431 | Lack of activity. | |||
Mutagenesis | 450 | No change in activity. | |||
Mutagenesis | 468 | No change in activity. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000423661 | 1-477 | Na+/H+ antiporter NhaD | ||
Structure
Sequence
- Sequence statusComplete
- Length477
- Mass (Da)52,378
- Last updated2007-06-12 v1
- MD5 Checksum39417C489F1E465E738DC93A1073DEDD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF331042 EMBL· GenBank· DDBJ | AAG48354.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000626 EMBL· GenBank· DDBJ | ABQ19159.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001236 EMBL· GenBank· DDBJ | ACP11869.1 EMBL· GenBank· DDBJ | Genomic DNA |