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A0A7X7H3M4 · A0A7X7H3M4_9GAMM

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site299Mg2+ (UniProtKB | ChEBI); catalytic
Binding site342Mg2+ (UniProtKB | ChEBI); catalytic
Binding site400Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site403Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      GX667_01130

Organism names

  • Taxonomic identifier
  • Strain
    • AS07pgkLD_132
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Lysobacteraceae

Accessions

  • Primary accession
    A0A7X7H3M4

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain39-117S1 motif
Region400-403Required for zinc-mediated homotetramerization and catalytic activity
Region527-546Disordered
Compositional bias565-590Basic and acidic residues
Region565-1026Disordered
Compositional bias596-622Polar residues
Compositional bias641-778Basic and acidic residues
Compositional bias788-857Basic and acidic residues
Compositional bias871-968Basic and acidic residues
Compositional bias971-995Polar residues
Compositional bias996-1014Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,026
  • Mass (Da)
    117,370
  • Last updated
    2021-06-02 v1
  • MD5 Checksum
    92DCE6205EC376527043AA9815C67C90
MKRMLINATQEEEMRVALVNGQHLYDLDIENIANRQKKSNIYKGTITRVEPSLEACFVNYGVERHGFLPFKEIAREYLAESNNQNAPLKDLIKEGTEVIVQVERDERGNKGAALTTYVSLAGRYLVLMPNNPRAGGVSRRISGKNRADLREQMEDLEVPEGMGLIVRTAGMGRVAEELQWDLNYLVMLWEAIQNASKEPAPFLIFQESNIIVRALRDYYRGDIGEILVDNEEVYKEAENFIDNIMPQIRDKIKLYKDNIPLFTRFQVESQIQTAYQRDVKLPSGGALVFDYTEALVSIDINSGRSTKGADIEETALNTNLEAAEEIARQLKLRDLGGLIVIDFIDMIETKHQREVENRFHDALASDRARIQVGQISRFGLLEMSRQRLRASLDETSHISCPRCDGQGTIRNTKSLALAILRLIEEECLKEKTAEIIVQVPIDIAIYLLNEKRDDMLDIETRLETRVIIIPNKEMDTPHFNITRYRTNDSGTIGQDSSTHVVVEEGTTEDLRNKALNLNRQILSAPAVTGIKPTTPPPTEVREGAEKPTVLQTIISFLKDLFDKKEEKSGKKEAKKGDNAKEDGKNKRRNQQNERRNNRRNNNNANRNRNVRMSPNRNNTIETVERNVDQKAEFIPAEENQAQDNNRRDNRRDNRNDERNDNRNQRQNRRDRQDNQNRQERNDARSERGDNQKGERQDNRSNRQDRDNRNDNRNDNRQNQNERRNRRDDRRDDRQEGESRDNDRRNERNDRNEERQERDNRNDNRQNNRRNRQNDRNNEANEQESNANAPQKTERKGEVIVDVNGEKRTRKIRDGRPRIEETEAQAPETKGADKAPVEAVKETANEAPKADTPKAEDKAANANAEAPQANAADNAKKDDESRGNQERNRRPRQNRNERQERPQAEEKAVNAEKAETAEPAKTEAPKAETAKTEVKTEESSPAVKESSSKADTTKEVKAAEKKVDANDANADKSAQKPAQTNEKAPSAEPSQSDAPEAKSSSHDERTPEHAEKPAQNAETPENSKPQQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias565-590Basic and acidic residues
Compositional bias596-622Polar residues
Compositional bias641-778Basic and acidic residues
Compositional bias788-857Basic and acidic residues
Compositional bias871-968Basic and acidic residues
Compositional bias971-995Polar residues
Compositional bias996-1014Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAAZCI010000030
EMBL· GenBank· DDBJ
NLD08175.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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