A0A7C9RCG2 · A0A7C9RCG2_9BRAD
- ProteinReplicative DNA helicase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids499 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The main replicative DNA helicase, it participates in initiation and elongation during chromosome replication. Travels ahead of the DNA replisome, separating dsDNA into templates for DNA synthesis. A processive ATP-dependent 5'-3' DNA helicase it has DNA-dependent ATPase activity.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | primosome complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Biological Process | DNA replication, synthesis of primer | |
Biological Process | DNA unwinding involved in DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplicative DNA helicase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Afipia
Accessions
- Primary accessionA0A7C9RCG2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
Homohexamer.
Structure
Sequence
- Sequence statusComplete
- Length499
- Mass (Da)54,917
- Last updated2021-02-10 v1
- MD5 Checksum80205C954EB7618D0D01EEA404440FC2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAAMRR010000083 EMBL· GenBank· DDBJ | NGX93963.1 EMBL· GenBank· DDBJ | Genomic DNA |