A0A6J2PKV2 · A0A6J2PKV2_COTGO

Function

function

Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine. Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP. Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency. Through these activities regulates the purine nucleoside/nucleotide pools within the cell.

Catalytic activity

  • inosine + AMP = IMP + adenosine
  • inosine + CMP = cytidine + IMP
  • GMP + H2O = guanosine + phosphate
    This reaction proceeds in the forward direction.
  • inosine + GMP = guanosine + IMP
  • IMP + H2O = inosine + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.99 (UniProtKB | ENZYME | Rhea)
  • XMP + H2O = xanthosine + phosphate
    This reaction proceeds in the forward direction.
  • a 2'-deoxyribonucleoside + a ribonucleoside 5'-phosphate = a ribonucleoside + a 2'-deoxyribonucleoside 5'-phosphate
    EC:2.7.1.77 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.5 (UniProtKB | ENZYME | Rhea)
  • dGMP + H2O = 2'-deoxyguanosine + phosphate
    This reaction proceeds in the forward direction.
  • dGMP + inosine = 2'-deoxyguanosine + IMP
  • dIMP + H2O = 2'-deoxyinosine + phosphate
    This reaction proceeds in the forward direction.
  • dIMP + inosine = 2'-deoxyinosine + IMP
  • inosine + UMP = uridine + IMP

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site52Nucleophile
Binding site52Mg2+ (UniProtKB | ChEBI)
Active site54Proton donor
Binding site54GMP (UniProtKB | ChEBI)
Binding site351Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function5'-nucleotidase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functiontransferase activity
Biological ProcessGMP metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytosolic purine 5'-nucleotidase
  • EC number
  • Alternative names
    • Cytosolic nucleoside phosphotransferase 5'N

Gene names

    • Name
      nt5c2

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Neoteleostei > Acanthomorphata > Eupercaria > Perciformes > Notothenioidei > Bovichtidae > Cottoperca

Accessions

  • Primary accession
    A0A6J2PKV2

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region531-562Disordered
Compositional bias548-562Acidic residues

Sequence similarities

Belongs to the 5'(3')-deoxyribonucleotidase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    562
  • Mass (Da)
    65,198
  • Last updated
    2020-10-07 v1
  • Checksum
    46F01AF7841AC5A4
MTTSWSDRLQNYADLPANMDGVTMKKYRREPYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFVYDPSFPTRGLVFDTMYGNLLKVDAYGNILVCVHGFNFLRGPEIRERYPNKFIQRDDTERFYILNTLFNLPETYLFACLVDFFSNCDRYTSCESGFKNGDLFMSYKSMFQDVRDAVDWVHFKGTLKEKTVENLEKYVVRDGKLPLLLSRMNEVAKVFLATNSDYKYTDKIMNYLFDFPHGPKPGTSHRPWQSYFDLILADARKPLFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDIVYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQGLDIFLAELYKHLDSSSNERPDISTIQRRVKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHAHVDIDMESPLATRNRTHCSDCKDLECKRNQLTRSFSEIKPPNLFPQTPQEITHCHDEDDDEEEEEEEEE

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A6J2PMZ6A0A6J2PMZ6_COTGOnt5c2400

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias548-562Acidic residues

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

Disclaimer

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