A0A6J2LL45 · A0A6J2LL45_9CHIR

Function

function

Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine. Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP. Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency. Through these activities regulates the purine nucleoside/nucleotide pools within the cell.

Catalytic activity

  • inosine + AMP = IMP + adenosine
  • inosine + CMP = cytidine + IMP
  • GMP + H2O = guanosine + phosphate
    This reaction proceeds in the forward direction.
  • inosine + GMP = guanosine + IMP
  • IMP + H2O = inosine + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.99 (UniProtKB | ENZYME | Rhea)
  • XMP + H2O = xanthosine + phosphate
    This reaction proceeds in the forward direction.
  • a 2'-deoxyribonucleoside + a ribonucleoside 5'-phosphate = a ribonucleoside + a 2'-deoxyribonucleoside 5'-phosphate
    EC:2.7.1.77 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.5 (UniProtKB | ENZYME | Rhea)
  • dGMP + H2O = 2'-deoxyguanosine + phosphate
    This reaction proceeds in the forward direction.
  • dGMP + inosine = 2'-deoxyguanosine + IMP
  • dIMP + H2O = 2'-deoxyinosine + phosphate
    This reaction proceeds in the forward direction.
  • dIMP + inosine = 2'-deoxyinosine + IMP
  • inosine + UMP = uridine + IMP

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site23Nucleophile
Binding site23Mg2+ (UniProtKB | ChEBI)
Active site25Proton donor
Binding site25GMP (UniProtKB | ChEBI)
Binding site322Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function5'-nucleotidase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functiontransferase activity
Biological Processadenosine metabolic process
Biological ProcessGMP metabolic process
Biological ProcessIMP metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytosolic purine 5'-nucleotidase
  • EC number
  • Alternative names
    • Cytosolic nucleoside phosphotransferase 5'N

Gene names

    • Name
      NT5C2

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Chiroptera > Yangochiroptera > Phyllostomidae > Phyllostominae > Phyllostomus

Accessions

  • Primary accession
    A0A6J2LL45

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region509-530Disordered

Sequence similarities

Belongs to the 5'(3')-deoxyribonucleotidase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    530
  • Mass (Da)
    61,150
  • Last updated
    2020-10-07 v1
  • Checksum
    9988ADC7447EF472
MSQEGVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEVESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEE

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A6J2LIT2A0A6J2LIT2_9CHIRNT5C2559
A0A7E6DWL4A0A7E6DWL4_9CHIRNT5C2527
A0A7E6DVC8A0A7E6DVC8_9CHIRNT5C2368
A0A7E6DVT5A0A7E6DVT5_9CHIRNT5C2586

Keywords

Sequence databases

Similar Proteins

Disclaimer

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