A0A5K7S3F1 · A0A5K7S3F1_9BACT
- ProteinFormate-dependent phosphoribosylglycinamide formyltransferase
- GenepurT
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids385 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.
Catalytic activity
- N1-(5-phospho-beta-D-ribosyl)glycinamide + formate + ATP = N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H+
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 7-8 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | |||
Binding site | 67 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | |||
Binding site | 99 | ATP (UniProtKB | ChEBI) | |||
Binding site | 140 | ATP (UniProtKB | ChEBI) | |||
Binding site | 145-150 | ATP (UniProtKB | ChEBI) | |||
Binding site | 180-183 | ATP (UniProtKB | ChEBI) | |||
Binding site | 188 | ATP (UniProtKB | ChEBI) | |||
Binding site | 253 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 265 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 272 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | |||
Binding site | 343 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | |||
Binding site | 350-351 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | ligase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | phosphoribosylglycinamide formyltransferase 2 activity | |
Molecular Function | phosphoribosylglycinamide formyltransferase activity | |
Biological Process | 'de novo' IMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFormate-dependent phosphoribosylglycinamide formyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Bacteroidia > Marinilabiliales > Prolixibacteraceae > Aquipluma
Accessions
- Primary accessionA0A5K7S3F1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length385
- Mass (Da)42,125
- Last updated2020-02-26 v1
- ChecksumC564272B0DFEAFC7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP018694 EMBL· GenBank· DDBJ | BBE15874.1 EMBL· GenBank· DDBJ | Genomic DNA |