A0A5K7S3F1 · A0A5K7S3F1_9BACT

  • Protein
    Formate-dependent phosphoribosylglycinamide formyltransferase
  • Gene
    purT
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.

Features

Showing features for binding site.

138550100150200250300350
TypeIDPosition(s)Description
Binding site7-8N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site67N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site99ATP (UniProtKB | ChEBI)
Binding site140ATP (UniProtKB | ChEBI)
Binding site145-150ATP (UniProtKB | ChEBI)
Binding site180-183ATP (UniProtKB | ChEBI)
Binding site188ATP (UniProtKB | ChEBI)
Binding site253Mg2+ (UniProtKB | ChEBI)
Binding site265Mg2+ (UniProtKB | ChEBI)
Binding site272N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site343N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site350-351N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionligase activity
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylglycinamide formyltransferase 2 activity
Molecular Functionphosphoribosylglycinamide formyltransferase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Formate-dependent phosphoribosylglycinamide formyltransferase
  • EC number
  • Alternative names
    • 5'-phosphoribosylglycinamide transformylase 2
    • Formate-dependent GAR transformylase
    • GAR transformylase 2
      (GART 2
      )
    • Non-folate glycinamide ribonucleotide transformylase
    • Phosphoribosylglycinamide formyltransferase 2

Gene names

    • Name
      purT
    • ORF names
      AQPE_0010

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • MeG22
  • Taxonomic lineage
    Bacteria > Bacteroidota > Bacteroidia > Marinilabiliales > Prolixibacteraceae > Aquipluma

Accessions

  • Primary accession
    A0A5K7S3F1

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain104-295ATP-grasp

Sequence similarities

Belongs to the PurK/PurT family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    385
  • Mass (Da)
    42,125
  • Last updated
    2020-02-26 v1
  • Checksum
    C564272B0DFEAFC7
MLLGSGELGKEFVIAAKRLGQTVIAVDSYAGAPAMQVADQFEVINMLDGAELDRIVDKHRPDLIVPEIEAIRTERFYDYEKAGIQVVPSAKAANFTMNRKAIRDLAAQELGLKTANYRYSKTFEELKAAVAEVGMPCVVKPLMSSSGKGQSTIKSEADFEKAWNTASDKGRGDLNEVIVEAFVPFLSEITLLTVTQKNGETLFCAPIGHRQERGDYQESWQPVFLPEGFEEEARRMAAMVTKALGGVGIWGVEFFIVEEGVVFSELSPRPHDTGMVTLAGTQNLSEFELHCRAVLGLPIPEIRQEKAGASAVILSGIETISPEYAGIDLVCQNSHADVRIFGKETARIHRRMGVVVVNAELGTDVLELKQTARNLADLVEVKPSK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP018694
EMBL· GenBank· DDBJ
BBE15874.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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