A0A5C7UT82 · A0A5C7UT82_9BURK
- ProteinN-acetylmuramic acid 6-phosphate etherase
- GenemurQ
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids293 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activity
- N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + (R)-lactate
Pathway
Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.
Amino-sugar metabolism; N-acetylmuramate degradation.
Cell wall biogenesis; peptidoglycan recycling.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 79 | Proton donor | |||
Active site | 110 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbohydrate derivative binding | |
Molecular Function | carbon-oxygen lyase activity | |
Molecular Function | ether hydrolase activity | |
Biological Process | 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process | |
Biological Process | amino sugar catabolic process | |
Biological Process | N-acetylmuramic acid catabolic process | |
Biological Process | peptidoglycan turnover |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylmuramic acid 6-phosphate etherase
- EC number
- Short namesMurNAc-6-P etherase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Sphaerotilaceae > Roseateles
Accessions
- Primary accessionA0A5C7UT82
Proteomes
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length293
- Mass (Da)29,881
- Last updated2019-11-13 v1
- MD5 ChecksumD1ABC91A3EBBF48A16989604E97A0F35
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
SSFQ01000322 EMBL· GenBank· DDBJ | TXI15735.1 EMBL· GenBank· DDBJ | Genomic DNA |