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A0A512IGU9 · A0A512IGU9_9MICC

  • Protein
    Ribokinase
  • Gene
    rbsK
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.

Activity regulation

Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.

Pathway

Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site340-342substrate
Binding site368-372substrate
Binding site463substrate
Binding site504ATP (UniProtKB | ChEBI)
Binding site523-528ATP (UniProtKB | ChEBI)
Binding site552K+ (UniProtKB | ChEBI)
Binding site555-556ATP (UniProtKB | ChEBI)
Active site556Proton acceptor
Binding site556substrate
Binding site580ATP (UniProtKB | ChEBI)
Binding site586K+ (UniProtKB | ChEBI)
Binding site589K+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpurine nucleosidase activity
Molecular Functionribokinase activity
Molecular Functionuridine nucleosidase activity
Biological ProcessD-ribose catabolic process
Biological Processpurine nucleoside catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribokinase
  • EC number
  • Short names
    RK

Gene names

    • Name
      rbsK
    • ORF names
      KTU01_30110

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • NBRC 107627
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Micrococcales > Micrococcaceae > Kocuria

Accessions

  • Primary accession
    A0A512IGU9

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain10-305Inosine/uridine-preferring nucleoside hydrolase
Domain333-593Carbohydrate kinase PfkB
Region590-617Disordered

Sequence similarities

Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.
Belongs to the carbohydrate kinase pfkB family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    617
  • Mass (Da)
    63,232
  • Last updated
    2019-10-16 v1
  • MD5 Checksum
    57744DF39E1FFCC3569F586BE993E7AB
MTDPAARPLYMDCDTGIDDAVALAYLVATPSVDLRGIGTVSGNTDAGTAARSTVDLLALLDRSDIPVAVGARDPLRDRFGGGAPAVHGGNGTGDVELPRTAAEPVTEAADAMLIRLAHEHPGELHVLATGPLTNLARALRQDPALPGLVAGVTVMGGAALVPGNRTPVAEANILHDPEAAAEVLAAAWEVTLVPLDVTMDHRLDEDQRQQLAAVDHPALPPLAAMLERYAEFYTGVFGRPLSALHDPLAAALAAGTVEPALAPRVTVQVDTTDGPGRGQTICDLRGRFTGYPAAAGARCRVVLELAEDFAPHLLNTLRRLAPAAPAEPSRAALTVVGSINVDLTAVCERLPAPGETVIGADLQRQPGGKGANQAVAAARLAGRARMIGAVGDDPDGGLLLRHLAAAGVDTTGVRRVPAPTGTALITVDRHGENQITVCAGANAEVSLEDVSFAPEDVVLCQLEIELDTVCETARRTPGFFALNAAPARPLPAELVERCDLVIVNETEYARIPELADAELVAVTYGAQGSALYAHGRQIASAPAHEVAVVSTVGAGDAFCAALVLALAAGLDHETALRAANAVGAHAVGDASSQPEFSPLEHYLPSPSDGGTPSAGPA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BJZS01000100
EMBL· GenBank· DDBJ
GEO96888.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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