A0A485P362 · A0A485P362_LYNPA
- ProteinLanosterol 14-alpha demethylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids503 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis. Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis. Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL.
Catalytic activity
- 24,25-dihydrolanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H+This reaction proceeds in the forward direction.
CHEBI:28113 + 3 RHEA-COMP:11964 + 3 CHEBI:15379 = CHEBI:78904 + CHEBI:15740 + 3 RHEA-COMP:11965 + 4 CHEBI:15377 + 4 CHEBI:15378 - 24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxy-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- 32-hydroxy-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxo-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H+This reaction proceeds in the forward direction.
- 32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H+This reaction proceeds in the forward direction.
- 32-oxo-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H+This reaction proceeds in the forward direction.
- 32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H+This reaction proceeds in the forward direction.
- a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta14 steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H+This reaction proceeds in the forward direction.
- a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H+This reaction proceeds in the forward direction.
- a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
Cofactor
Pathway
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 449 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | cholesterol metabolic process | |
Biological Process | organic hydroxy compound biosynthetic process | |
Biological Process | small molecule biosynthetic process | |
Biological Process | steroid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLanosterol 14-alpha demethylase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Feliformia > Felidae > Felinae > Lynx
Accessions
- Primary accessionA0A485P362
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Microsome membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 25-44 | Helical | |||
Keywords
- Cellular component
Structure
Sequence
- Sequence statusComplete
- Length503
- Mass (Da)56,828
- Last updated2019-06-05 v1
- MD5 Checksum8C92FDC4F6CC1674B90264DB68FE3AF9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CAAGRJ010028562 EMBL· GenBank· DDBJ | VFV40160.1 EMBL· GenBank· DDBJ | Genomic DNA |