A0A3S5ET35 · A0A3S5ET35_9CORY

Function

function

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit.

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site26-29NADP+ (UniProtKB | ChEBI)
Binding site49NADP+ (UniProtKB | ChEBI)
Binding site52NADP+ (UniProtKB | ChEBI)
Binding site54NADP+ (UniProtKB | ChEBI)
Active site109
Binding site135NADP+ (UniProtKB | ChEBI)
Binding site192Mg2+ 1 (UniProtKB | ChEBI)
Binding site192Mg2+ 2 (UniProtKB | ChEBI)
Binding site196Mg2+ 1 (UniProtKB | ChEBI)
Binding site228Mg2+ 2 (UniProtKB | ChEBI)
Binding site232Mg2+ 2 (UniProtKB | ChEBI)
Binding site253substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionisomerase activity
Molecular Functionketol-acid reductoisomerase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNADP binding
Biological Processisoleucine biosynthetic process
Biological Processvaline biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ketol-acid reductoisomerase (NADP(+))
  • EC number
  • Short names
    KARI
  • Alternative names
    • Acetohydroxy-acid isomeroreductase
      (AHIR
      )
    • Alpha-keto-beta-hydroxylacyl reductoisomerase

Gene names

    • Name
      ilvC
    • ORF names
      NCTC934_01033

Organism names

  • Taxonomic identifier
  • Strain
    • NCTC934
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Corynebacteriaceae > Corynebacterium

Accessions

  • Primary accession
    A0A3S5ET35

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain, coiled coil.

Type
IDPosition(s)Description
Domain1-183KARI N-terminal Rossmann
Domain184-329KARI C-terminal knotted
Coiled coil289-316

Sequence similarities

Belongs to the ketol-acid reductoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    337
  • Mass (Da)
    36,518
  • Last updated
    2019-04-10 v1
  • MD5 Checksum
    4C8BFF0B7F1C0E800916B2BC95AAB54A
MAIDTYYDDDADLSIIQGRKVAVIGYGSQGHAHSQNLRDSGVEVVIGLREGSKSAAKAEEAGFEVKSVAEASKWADVVMLLAPDTSQKEIFEEHIAPNLEDGNALLFGHGLNIHFKLIEPAASVTVGMVAPKGPGHLVRRQFVDGKGVPCLIATEQDPKNEGHDLTLSYAAAIGGARAGVIPTTFEAETVTDLFGEQAVLCGGVEFLIRNGFEVLTEAGYEPEMAYFEVLHEMKLIVDLMFEGGIANMNYSVSDTAEFGGYISGPRIINADTKQRMKDVLSDIQDGTFTKRLLANVEGGNKELEDLRDEFAQHEIEKTGTKLRDLMSWVKNPLDATA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LR134408
EMBL· GenBank· DDBJ
VEH72758.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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