A0A327QMK1 · A0A327QMK1_9FLAO

  • Protein
    Queuine tRNA-ribosyltransferase
  • Gene
    tgt
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site92Proton acceptor
Binding site92-96substrate
Binding site146substrate
Binding site194substrate
Binding site221substrate
Active site271Nucleophile

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA wobble guanine modification
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • ORF names
      LV92_04266

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DSM 23522
  • Taxonomic lineage
    Bacteria > Bacteroidota > Flavobacteriia > Flavobacteriales > Flavobacteriaceae > Arenibacter

Accessions

  • Primary accession
    A0A327QMK1

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain13-372tRNA-guanine15 transglycosylase-like
Region252-258RNA binding
Region276-280RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    376
  • Mass (Da)
    43,187
  • Last updated
    2018-10-10 v1
  • Checksum
    DEEFBCE2DC9FA6ED
MKFTLQHSDSRTKARAAELITDHGKIETPIFMPVGTVASVKGVHQKELREEINPDIILGNTYHLYLRPKTEILKQAGGLHKFMNWDRNILTDSGGYQVYSLSDNRKIKEEGVKFKSHIDGSYHVFTPENVMEIQRVIGADIIMAFDECTPYPCDYNYAKRSMHMTHRWLDRCIKHLEKTPFTYDFEQTFFPIVQGSTYKDLRQQSAEYIAGVGAEGNAIGGLSVGEPAEEMYAMTEVVCEILPEDKPRYLMGVGTPINILENIALGIDMFDCVMPTRNARNGMLFTAHGTINIKNKKWEDDFSPLDEMGTTFVDHEYSKAYLRHLFAANEYLGKQIATIHNLGFYMWLVREARKHILAGDFYEWKSVMVKQMDKRL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QLLN01000012
EMBL· GenBank· DDBJ
RAJ05560.1
EMBL· GenBank· DDBJ
Genomic DNA

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Disclaimer

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