A0A2I1C3W7 · NSRE_ASPN1

Function

function

Decarboxylase; part of the gene cluster that mediates the biosynthesis of the tetrahydroxanthone dimer neosartorin, which exhibits antibacterial activity (PubMed:30394754, PubMed:32105084, PubMed:33891392).
The two different monomeric units appear to be synthesized by the same set of enzymes, among which the Baeyer-Villiger monooxygenase nsrF is the key enzyme for the divergence of the biosynthetic routes (PubMed:32105084).
The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase nsrB (PubMed:32105084).
The atrochrysone carboxyl ACP thioesterase nsrC then breaks the thioester bond and releases the atrochrysone carboxylic acid from AacuL (PubMed:32105084).
Atrochrysone carboxylic acid is decarboxylated by the decarboxylase nsrE, and oxidized by the anthrone oxygenase nsrD to yield emodin (PubMed:32105084).
Emodin is then reduced to emodin hydroquinone by the oxidoreductase nsrR (PubMed:32105084).
A-ring reduction by the short chain dehydrogenase nsrJ, dehydration by the scytalone dehydratase-like protein nsrI and probable spontaneous re-oxidation, results in overall deoxygenation to chrysophanol (PubMed:32105084).
The Baeyer-Villiger monooxygenase nsrF accepts chrysophanol as a substrate to insert one oxygen atom at two different positions to yield the precursors of both monomric units (PubMed:30394754, PubMed:32105084, PubMed:33891392).
NsrF is promiscuous/flexible in interacting with the 2 (non methylated and methylated) aromatic rings of chrysophanol, thus diverging the biosynthetic pathway at this point (PubMed:30394754, PubMed:32105084, PubMed:33891392).
After the hydrolysis of the lactones, methylesterification by the methyltransferase nsrG yields respectively moniliphenone and 2,2',6'-trihydroxy-4-methyl-6-methoxya-cyldiphenylmethanone (PubMed:30394754, PubMed:32105084).
The next steps are the hydroxylation by the FAD-dependent monooxygenase nsrK, followed by isomerization by the monooxygenase nsrQ (PubMed:32105084).
The short chain dehydrogenase/reductase nsrO then catalyzes the C-5 ketoreduction to give the xanthone skeleton of blennolide C and 5-acetylblennolide A (PubMed:32105084).
The acetyltransferase nsrL has a strict substrate specificity and uses only blennolide A but not blennolide C to yield 5-acetylblennolide A as the single-acetylated product (PubMed:30394754).
In the final step of the biosynthesis, the heterodimerization of the 2 xanthones, blennolide C and 5-acetylblennolide A, is catalyzed by the cytochrome P450 monooxygenase nsrP (PubMed:30394754).
NsrP can utilize at least three different xanthones as its substrates to perform the dimerization reaction (PubMed:30394754).

Catalytic activity

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functionlyase activity
Molecular Functionoxidoreductase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Decarboxylase nsrE
  • EC number
  • Alternative names
    • Neosartorin biosynthesis cluster protein E

Gene names

    • Name
      nsrE
    • ORF names
      P174DRAFT_372388

Organism names

  • Taxonomic identifier
  • Strain
    • IBT 16806
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Fumigati

Accessions

  • Primary accession
    A0A2I1C3W7

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004534351-151Decarboxylase nsrE

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain31-126EthD

Sequence similarities

Belongs to the tpcK family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    151
  • Mass (Da)
    17,556
  • Last updated
    2018-02-28 v1
  • Checksum
    5D29020B5C3BE3F4
MASSTHPEMPELSGSYENKYLCLTICGYRKAGMTEEDYHNHMVHISAPMTKHLMVKYGIRRWTQIHNQAATRALMAELFDPQMANVADFDCFSQVVFQNVEDYKRMKQDPWYQEHLVGDHENFADTKRTLVTIGWIEEFVRDGEAVDGFKS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MSZS01000005
EMBL· GenBank· DDBJ
PKX92305.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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