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A0A2A3N154 · A0A2A3N154_9SPHN

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispDF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

Type
IDPosition(s)Description
Site19Transition state stabilizer
Site26Transition state stabilizer
Site155Positions MEP for the nucleophilic attack
Site210Positions MEP for the nucleophilic attack
Binding site237a divalent metal cation (UniProtKB | ChEBI)
Binding site237-2394-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site239a divalent metal cation (UniProtKB | ChEBI)
Site263Transition state stabilizer
Binding site263-2644-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site271a divalent metal cation (UniProtKB | ChEBI)
Binding site285-2874-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site361-3644-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site362Transition state stabilizer
Binding site3684-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3714-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispDF
    • ORF names
      SxD43FB_01015

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • D43FB
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingobium

Accessions

  • Primary accession
    A0A2A3N154

Proteomes

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-2302-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Domain230-3832-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region231-3892-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
Belongs to the IspF family.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    389
  • Mass (Da)
    40,882
  • Last updated
    2017-12-20 v1
  • MD5 Checksum
    28689CC95E42188C3D1C3A6ADDC97DD9
MIDNSKTVALLVAAGQGNRAGGDTPKQFRLVASKAVLAHAYDALAAHGAIDAIHIVLGAGQEDMARKLLGDRVASFIEGADSRRGSVRAGLESIAATGGAARILIHDAARPFLPGVVIDRLLGALGEAEGAIPVLPVADTLVRGMDGRMADGVPRDALFRVQTPQAFRFDTILAAHQAWDIAREATDDAQILRAVGHDAILVDGDEGLEKLTYPQDFARAEARLAPIMTVRVGMGYDVHRLATDELLWLGGVLVPHDRGLAGHSDADVALHAIVDALLGALAEGDIGSHFPPSDAQWRGASSDRFLAYAGDRVTARGGRIDHVDLTIICEAPKIGPHRDAMRARIGTILAVPIDRVSVKATTTERLGFAGRREGIAAQAVATLSLPALS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NMUA01000001
EMBL· GenBank· DDBJ
PBN45471.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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