A0A238FA18 · A0A238FA18_9BASI
- ProteinNADPH-dependent diflavin oxidoreductase 1
- GeneTAH18
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids701 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.
Catalytic activity
- 2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP+ + H+
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 153-162 | FMN (UniProtKB | ChEBI) | |||
Binding site | 188 | FMN (UniProtKB | ChEBI) | |||
Binding site | 410 | FAD (UniProtKB | ChEBI) | |||
Binding site | 440-443 | FAD (UniProtKB | ChEBI) | |||
Binding site | 474-477 | FAD (UniProtKB | ChEBI) | |||
Binding site | 516 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 571-572 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 609-613 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 700 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | membrane | |
Cellular Component | mitochondrion | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Biological Process | iron-sulfur cluster assembly |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent diflavin oxidoreductase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Pucciniomycotina > Microbotryomycetes > Microbotryales > Microbotryaceae > Microbotryum
Accessions
- Primary accessionA0A238FA18
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Relocalizes to mitochondria after H2O2 exposure.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 581-599 | Helical | |||
Keywords
- Cellular component
Interaction
Subunit
Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 23-206 | Flavodoxin-like | |||
Domain | 255-502 | FAD-binding FR-type | |||
Region | 658-679 | Disordered | |||
Sequence similarities
Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length701
- Mass (Da)80,519
- Last updated2017-11-22 v1
- Checksum816EA738D532D549
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FMSP01000003 EMBL· GenBank· DDBJ | SCV67968.1 EMBL· GenBank· DDBJ | Genomic DNA |