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A0A1Y3WYC8 · A0A1Y3WYC8_9FIRM

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site137-138dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site157
Active site157Nucleophile
Binding site177-180dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site180(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site188dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site218-220dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site275(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      B5G06_12920

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • An52
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Flavonifractor

Accessions

  • Primary accession
    A0A1Y3WYC8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-267Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    276
  • Mass (Da)
    31,932
  • Last updated
    2017-08-30 v1
  • MD5 Checksum
    9C80EB4FC0CCC35DD8DB9A70AADDEB1D
MSRADELFIQNCKDILAHGVWDTDQEVRPRWEDGTPAHTVKKFCIVNRYDLREEFPVQTIRKMAFKSAVDELLWIWQKKSNNIHDLNSHIWDAWADESGSIGKAYGYQLGVKHQYAEGMFDQVDRVLYDLKHNPASRRIMTNLYNHHDLHEMALYPCAYSMTFNVSGKTLNAILNQRSQDMLTANGWNVMQYAALVHMMAQVSGLEVGELVHVIADAHIYDRHVPVVEEIIARTPYEAPKLWMDPSVTDFYAFTKDSFKLEGYQFHTLDQPIPVAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NFIB01000044
EMBL· GenBank· DDBJ
OUN78292.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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