A0A1X0NHV5 · A0A1X0NHV5_9TRYP
- Proteinthymine dioxygenase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids824 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions.
Catalytic activity
- thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | thymine dioxygenase activity | |
Biological Process | base J metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namethymine dioxygenase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma
Accessions
- Primary accessionA0A1X0NHV5
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Subunit
Monomer. Binds to DNA as a monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 122-278 | 2OGFeDO JBP1/TET oxygenase | |||
Domain | 411-573 | Thymine dioxygenase JBP1 DNA-binding | |||
Sequence similarities
Belongs to the TET family. JBP1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length824
- Mass (Da)93,288
- Last updated2017-07-05 v1
- ChecksumFBCB6B5CF80BD0B4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
NBCO01000046 EMBL· GenBank· DDBJ | ORC84342.1 EMBL· GenBank· DDBJ | Genomic DNA |