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A0A1H7PEK7 · A0A1H7PEK7_9SPHN

  • Protein
    Ribonuclease E
  • Gene
    rne
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

1923100200300400500600700800900
TypeIDPosition(s)Description
Binding site406Mg2+ (UniProtKB | ChEBI); catalytic
Binding site449Mg2+ (UniProtKB | ChEBI); catalytic
Binding site507Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site510Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      SAMN05216382_1827

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • JS21-1
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingomonas

Accessions

  • Primary accession
    A0A1H7PEK7

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, compositional bias, region.

Type
IDPosition(s)Description
Domain38-222S1 motif
Compositional bias114-154Acidic residues
Region114-178Disordered
Compositional bias157-178Basic and acidic residues
Region507-510Required for zinc-mediated homotetramerization and catalytic activity
Compositional bias612-628Acidic residues
Region612-923Disordered
Compositional bias629-647Basic and acidic residues
Compositional bias658-674Basic and acidic residues
Compositional bias675-694Acidic residues
Compositional bias767-782Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    923
  • Mass (Da)
    101,553
  • Last updated
    2017-11-22 v1
  • MD5 Checksum
    446C3E9B2EAC41075FC25F5EC3FA8EA2
MTTRMLIDARHREETRVAVVKGNRIEEFDFESAERKQLKGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQIPKEDRDALLREEAEHAAEEAKLRAELDAEDAGFDEDDDAHHDDDDAHELDDDHADEDDAHDDHVDDAGGDDDADGASENAGGRRRKGRRSNGDDQVEALRQRRMNLRRRYKIQDVIRRRQVLLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTSHGGGISRKISNAGDRKRLKAIMADLELPSSMGCIVRTAGLQRTKTEIKRDFDYLARLWDEIRDKTLSSSAPALVYGDSDLMKRAIRDIYNKDIDEVIVEGDDGYRQAKDFMRLLMPTHARKVKHYSDPIPLYQRAGVEDQLAAMYHPVVQLKSGGYLVINPTEALVSIDINSGRSTREHNIEQTATATNLEAAQEIARQLRLRDMAGLVVIDFIDMDHGSNVRKVEKAMKEALKNDRARIQVGRISSFGLMEMSRQRLRTGVLEASTRQCPHCEGTGLVRTASSAGLSALRLIEDEAARGRGSHLTLRCSQEAAFYVLNRKRGDIAEIEDRYGVQVDILADGEEEGARMSVEAAGPPPAYAPKFDAPMIEVEEDLEDEYEVEEEEEAVEEEAPAERGQRERDSEDREGGRRKRRRRGRRNRGRGEGEDSHADAAGEAVEADADEADAADESTSVDEADDGAREERGEGNGRRRRRNRRGRRRDGQGGASDETQVAGEAVSEPAPEEQPVEASSEPVIEEPVAEVEAAPEPAAKPKTRRRPRARAADATPDAPAAAEAEPATASVEEAPAADDASAAPEAPAKPKRTRRKKSDASAVEAGDAAPVTDGLPAPEVAEEAAPAKPKRTRRKKADAAPAEVEAVPQADAPSSADKAPVAVNAEPSGDDAQPASDGDASPRRGWWQRTFGN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias114-154Acidic residues
Compositional bias157-178Basic and acidic residues
Compositional bias612-628Acidic residues
Compositional bias629-647Basic and acidic residues
Compositional bias658-674Basic and acidic residues
Compositional bias675-694Acidic residues
Compositional bias767-782Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FNZZ01000003
EMBL· GenBank· DDBJ
SEL33844.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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