Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

A0A1E4G4K2 · A0A1E4G4K2_9HYPH

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site385Mg2+ (UniProtKB | ChEBI); catalytic
Binding site428Mg2+ (UniProtKB | ChEBI); catalytic
Binding site486Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site489Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      ABS76_23780

Organism names

Accessions

  • Primary accession
    A0A1E4G4K2

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, compositional bias, region.

Type
IDPosition(s)Description
Domain39-201S1 motif
Compositional bias94-109Basic and acidic residues
Region94-168Disordered
Compositional bias153-168Basic and acidic residues
Region486-489Required for zinc-mediated homotetramerization and catalytic activity
Region571-883Disordered
Compositional bias574-594Basic and acidic residues
Compositional bias595-610Acidic residues
Compositional bias648-662Acidic residues
Compositional bias741-760Basic and acidic residues
Compositional bias777-797Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    883
  • Mass (Da)
    97,369
  • Last updated
    2017-01-18 v1
  • MD5 Checksum
    7478E86252C9A2A72218FC113CCD0E0F
MATKRMLVDAIHPEETRIVVTAGNRLEEFDFESATRRQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPVADREALLREEEHHDDDHHDDDNGEDTVSLPEGVTEPDAEADPQDTEDASHIEQAPANTEPVESIGGDDALEEVPERRRQGPSRRNYKIQEVIKRRQVMLVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTARGGGISRKITNAADRKRLKEITSDLEVPQGMGVILRTAGASRTKAEVKRDFEYLMRLWESVRELTLKSQAPCLVYEEGSLIKRTIRDLYNKDIDEVLVAGDGAYREAKDFMKMIMPSHAKNVKPYGEDQPLFSKYNIETQLDQMFSPQVTLPSGGYLVINPTEALVSIDVNSGRSTKEHNIEDTALQTNLEAAEEVARQLRLRDLAGLIVIDFIDMMENRSNRAVERKLKDCLKDDRARIQVGRISHFGLMEMSRQRIRFGVVESSTHKCPICDGTGLVRSTESLALMIMRHIEDHVLRRHGQSINVRVPVEVALYILNFKRETLTALEARNQLSITITADGKLTGQQFAIEKGEARNSSYAEQRAAEHVRVDSAVIEDDIDEAVEEAEDEEHAASEAGDGNGRRRRRRRRRGGAERGENGQHQDVAAASGEDEAGDAETEAEDGESAAPAGEGNGEDEPRRRRRRGRRGGRRNRREGEENGEQVAAAEPEAEGEVSAAPEAVEAAPVVAEEPPAVVEAAPEPEAPVAEKPKRTRRKKVTETEESAPAETAPVEAVPAAEPVAEEKPKRKPRARKPKAETAETPATEAVAEAAPAPEPAAEAAAVETEAPAKTTRPRRTKKELPAEGIVVSSSTPAAAPAPAEGEGEAAAEPPKKKAGWWQRRLGLG

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias94-109Basic and acidic residues
Compositional bias153-168Basic and acidic residues
Compositional bias574-594Basic and acidic residues
Compositional bias595-610Acidic residues
Compositional bias648-662Acidic residues
Compositional bias741-760Basic and acidic residues
Compositional bias777-797Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MEEV01000032
EMBL· GenBank· DDBJ
ODT78415.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help