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A0A1C5RNJ3 · A0A1C5RNJ3_9CLOT

Function

function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site38Mg2+ (UniProtKB | ChEBI)
Binding site242Mg2+ (UniProtKB | ChEBI)
Binding site244Mg2+ (UniProtKB | ChEBI)
Binding site278Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2-isopropylmalate synthase activity
Molecular Functionacetyl-CoA C-acetyltransferase activity
Molecular Functionmagnesium ion binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-isopropylmalate synthase
  • EC number
  • Alternative names
    • Alpha-IPM synthase
    • Alpha-isopropylmalate synthase

Gene names

    • Name
      leuA
    • ORF names
      SAMEA3545261_01362
      , SAMEA3545398_02360

Organism names

  • Taxonomic identifier
  • Strains
    • 2789STDY5608853
    • 2789STDY5834935
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium > environmental samples

Accessions

  • Primary accession
    A0A1C5RNJ3

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain29-303Pyruvate carboxyltransferase
Region436-553Regulatory domain

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    553
  • Mass (Da)
    62,184
  • Last updated
    2016-11-02 v1
  • MD5 Checksum
    DB16C11376F5AF7C3D75DDC9EC823693
MNYKKYQKMYHPIPLEDRQWPNNEITHAPIWCSVDLRDGNQALYSPMTTEEKIEFFKFLVKLGFKEIEVGFPAASHTEFEFVRRLIDENLIPDDVTIQVLTQAREHIIKKTVEALKGAKNAIIHLYNSTSTLQREVVFRKSKEEIKQLAVDGAKLVMSLVDGQLDGNIRFEYSPESFTCTEPDYAAEVTNAVLDVFKPSETNKVIVNLPSTIEVATANVYADQIEYMCKHLNNREHLVISVHAHNDRGTGVASSELALLAGADRVEGTIFGNGERTGNTDVITVALNMFCQGIDPELHLENIDEIIEVYEKYNRLPINPRHPYAGEMAYVAFSGSHQDAIKKSMDKFGSSPSNKWANPYLTIDPTDIGRKYEPILINSQSGKGGVSYIMENKYGYTLPKPMLAEFSSLITDMSDEKKTVLTNHEIHQAFKDTYVNVAEPIKTRFYRSTEEDDCTILSATIEKDGKVTSIEGKGNGPLDALCNGLRQFLGQQFEICNYTEHALTRSSNSRAATYIEINDHNHHKTYWGAGVDANINTASIYALISAVNRMLADN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FMER01000008
EMBL· GenBank· DDBJ
SCH63018.1
EMBL· GenBank· DDBJ
Genomic DNA
FMGK01000020
EMBL· GenBank· DDBJ
SCJ22834.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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