A0A1C5RNJ3 · A0A1C5RNJ3_9CLOT
- Protein2-isopropylmalate synthase
- GeneleuA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids553 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity
- 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H+
Cofactor
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 38 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 242 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 244 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 278 | Mg2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | acetyl-CoA C-acetyltransferase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-isopropylmalate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium > environmental samples
Accessions
- Primary accessionA0A1C5RNJ3
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 29-303 | Pyruvate carboxyltransferase | |||
Region | 436-553 | Regulatory domain | |||
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length553
- Mass (Da)62,184
- Last updated2016-11-02 v1
- MD5 ChecksumDB16C11376F5AF7C3D75DDC9EC823693
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FMER01000008 EMBL· GenBank· DDBJ | SCH63018.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FMGK01000020 EMBL· GenBank· DDBJ | SCJ22834.1 EMBL· GenBank· DDBJ | Genomic DNA |