A0A133PEH3 · A0A133PEH3_FUSNU

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site40(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site40dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site40isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site79(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site79dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site79isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site101[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site129(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site129dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site129isopentenyl diphosphate (UniProtKB | ChEBI)
Active site131Proton donor
Binding site168(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site196[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site224(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site224dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site224isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site225(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site225dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site225isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site226(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site226dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site226isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site268(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site268dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site268isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Molecular Functionnucleic acid binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      HMPREF3221_00051

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • MJR7757B
  • Taxonomic lineage
    Bacteria > Fusobacteriota > Fusobacteriia > Fusobacteriales > Fusobacteriaceae > Fusobacterium

Accessions

  • Primary accession
    A0A133PEH3

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, coiled coil.

TypeIDPosition(s)Description
Domain477-545S1 motif
Domain562-632S1 motif
Domain649-716S1 motif
Domain733-802S1 motif
Coiled coil796-823

Sequence similarities

Belongs to the IspH family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    827
  • Mass (Da)
    94,368
  • Last updated
    2016-06-08 v1
  • Checksum
    7ECF09B4CE5B3BCA
MEIIRAKYMGFCFGVLEAINVCNSLVEEKGKKYILGMLVHNKQVVEDMQRKGFKLVTEEELLQDVDSLEENDIVVIRAHGTSKKVHEKLKERKVKVFDATCIFVNKIRQEIEIANEKGYSILFMGDRNHPEVKGVISFADDIQIFESYEEATKLKIDLDKTYLLSTQTTLNKRKFEEVKKFFKENYKNVIIFDKICGATAVRQKAVEDLAVKVELMIVVGDTKSSNTKKLYEISKKLNDNTYLIENEEQLNLDIFSGKKVVGITAGASTPEETIMNIEKKIRGIYKMSNVNENQNEFSQMLEEFLPNQEKRVEGVIESMDQNFSYLDVPGERTAVRVRTDELKGYKVGDTVEVLITGLSEEDDDQEYITASRKKIEVEKNWEKIEDSFKNKTILDAEVTKKIKGGYLVQALLYPGFLPNSLSEIPDNEEKVVGKKIQVIVKDIKVDSKDKRNKKITYSVKDIKIAEQEKEFAALEVGQIVDCVVTEVLDFGLAVDINTLKGFIHISEVSWKRLDKLSDIYKVGDHIKAVIVSLDEAKRNVKLSIKKLEEDPWATVANEFKVNDEVEGTVTKVLPYGAFVEIKPGIEGLVHISDFSWTKKKVNVSDYVKEGEKVKVRITDLHPEDRKLKLGIKQLVANPWETAEKDFAVGTVIKGKIVEVKPFGIFVEIADGIDAFVHSSDYNWIGEETPKFEIGNEVELKITELDLNNKKIKGSLKALRKSPWEHVLEEYKVGTTVEKKIKTVADFGLFIELIKGIDGFIPTQFASKEFIKNIRDKFKEGDIVKAQVVEVNKDTQKIKLSIKKIEIEEEKREEREQIEKYSTSSSEE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LRPY01000005
EMBL· GenBank· DDBJ
KXA26977.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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