A0A0T6AK55 · A0A0T6AK55_9BACT

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site47L-glutamine (UniProtKB | ChEBI)
Binding site237L-glutamine (UniProtKB | ChEBI)
Binding site239L-glutamine (UniProtKB | ChEBI)
Active site264Nucleophile
Binding site265L-glutamine (UniProtKB | ChEBI)
Binding site268L-glutamine (UniProtKB | ChEBI)
Binding site306L-glutamine (UniProtKB | ChEBI)
Binding site308L-glutamine (UniProtKB | ChEBI)
Binding site309L-glutamine (UniProtKB | ChEBI)
Active site349
Active site351

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Molecular Functiontransferase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processglutamine metabolic process
Biological ProcessL-arginine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      XU13_C0002G0036

Organism names

Accessions

  • Primary accession
    A0A0T6AK55

Proteomes

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-188CPSase
Domain3-133Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    376
  • Mass (Da)
    40,757
  • Last updated
    2016-02-17 v1
  • MD5 Checksum
    38E68DBAAF84AF4CF268E7CBB4E5F2E1
MIRAALLALADGRVFRGESLGAEGEATGEIVFNTAMTGYQEIMTDPSYKGQIVVMTYPLIGNYGINDEDVESRRPWVEGFVVKEASPTPSSWRGRRTLDEYMREQRIVGIQGIDTRALTRHLRDHGAQEGVISTEELDPARLAAKAKAAPGLVGRDLVREVTISAPHGWDEATWDRVRGYREPAAARFKVVAFDSGIKRNILRQLVSLGCALTVVPASTAAAAVLEQKPDGVFLSNGPGDPEGVPYLVEAVRALLGKVPIFGICLGHQILGLAAGGSTYKLKFGHHGANHPVKDLATGKVEITAQNHGFAVDPMSVKAHGLDLSHVNLNDGTCEGLRHRELPVFSVQYHPEASPGPHDANYLFNRFVDLMRRTKGG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LDXP01000002
EMBL· GenBank· DDBJ
KRT75349.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice.
Help