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A0A0R2PWM6 · A0A0R2PWM6_9ACTN

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site51L-glutamine (UniProtKB | ChEBI)
Binding site224L-glutamine (UniProtKB | ChEBI)
Binding site226L-glutamine (UniProtKB | ChEBI)
Active site252Nucleophile
Binding site253L-glutamine (UniProtKB | ChEBI)
Binding site256L-glutamine (UniProtKB | ChEBI)
Binding site294L-glutamine (UniProtKB | ChEBI)
Binding site297L-glutamine (UniProtKB | ChEBI)
Active site336
Active site338

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      ABR67_01870

Organism names

Accessions

  • Primary accession
    A0A0R2PWM6

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-176CPSase
Domain4-137Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    360
  • Mass (Da)
    38,790
  • Last updated
    2016-01-20 v1
  • MD5 Checksum
    983B724DE9E95EC32357FEA765D040E7
MSVREGQLVLRDGSVFEGELIGAISPRGISSGEVVFHTGMTGYQEVISDPSYAGQIITFTTPHIGNYGTTQADNEAFRVHARGVIVREEARRHSNWRSDSSLDAYLQSMMVSGIGGIDTRRLTRVLRDTGAMAGAFGSADESTLLTAARNEVGTSGIKLVEQVTTQQPYVVGSGHYKVVAFDFGIKSTILRCLSEIATVTVVPASTTAEQVRLLAPDGVFLSNGPGDPEAVDGAPETIRDLVVGGVPVFGICLGHQLLSLALGGKTYKLPFGHHGANHPVRNLKTGSVEITSQNHNFCVDTNSLRDVADITHTNLNDMTNEGMKVRDARAFSVQYHPEAGPGPHDSRYLFDEFRQMMEAQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LIAZ01000157
EMBL· GenBank· DDBJ
KRO42447.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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