A0A0N0P8A0 · A0A0N0P8A0_LEPSE
- ProteinThymine dioxygenase JBP1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids824 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions.
Catalytic activity
- thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | thymine dioxygenase activity | |
Biological Process | base J metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThymine dioxygenase JBP1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Leishmaniinae > Leptomonas
Accessions
- Primary accessionA0A0N0P8A0
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Subunit
Monomer. Binds to DNA as a monomer.
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 107-263 | 2OGFeDO JBP1/TET oxygenase | |||
Domain | 397-558 | Thymine dioxygenase JBP1 DNA-binding | |||
Compositional bias | 541-558 | Basic and acidic residues | |||
Region | 541-571 | Disordered | |||
Sequence similarities
Belongs to the TET family. JBP1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length824
- Mass (Da)92,510
- Last updated2015-12-09 v1
- ChecksumF2FE54340830E9A0
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 541-558 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LJSK01000018 EMBL· GenBank· DDBJ | KPI89706.1 EMBL· GenBank· DDBJ | Genomic DNA |